Hayashi Naoki, Takahashi Kenji, Abe Yoshinao, Kashiwakura Ikuo
Department of Radiological Life Sciences, Hirosaki University Graduate School of Health Sciences, 66-1 Hon-cho, Hirosaki, Aomori 036-8564, Japan.
Life Sci. 2009 Apr 24;84(17-18):598-605. doi: 10.1016/j.lfs.2009.02.006. Epub 2009 Feb 11.
The potential of human mesenchymal stem cell-like stroma prepared from placental/umbilical cord blood for hematopoietic regeneration by X-irradiated hematopoietic stem cells is herein assessed.
Placental/umbilical cord blood-derived mesenchymal stem cell-like stromal cells were applied to a regenerative ex vivo expansion of X-irradiated human CD34(+) cells in a serum-free liquid culture supplemented with a combination of interleukine-3 plus stem cell factor plus thrombopoietin.
The total number of cells and of lineage-committed myeloid hematopoietic progenitor cells generated in the co-culture of both non-irradiated and X-irradiated cells with stromal cells was significantly higher than those in the stroma-free culture. In addition, the number of CD34(+) cells and CD34(+)/CD38(-) cells, immature hematopoietic stem/progenitor cells also increased more than the stroma-free culture. The stromal cells produced various types of cytokines, although there was little difference between the co-cultures of non-irradiated and X-irradiated cells with stromal cells. Furthermore, when X-irradiated cells came in contact with stromal cells for 16 h before cytokine stimulation, a similar degree of hematopoiesis was observed, thus suggesting the critical role of cell-to-cell interaction.
The present results showed the potential efficacy of human mesenchymal stem cell-like stroma for hematopoietic regeneration from irradiated hematopoietic stem/progenitor cells.
本文评估了由胎盘/脐带血制备的人间充质干细胞样基质对经X射线照射的造血干细胞进行造血再生的潜力。
将胎盘/脐带血来源的间充质干细胞样基质细胞应用于在添加白细胞介素-3、干细胞因子和血小板生成素组合的无血清液体培养中对经X射线照射的人CD34(+)细胞进行再生性体外扩增。
未照射和经X射线照射的细胞与基质细胞共培养所产生的细胞总数和定向谱系髓系造血祖细胞数量均显著高于无基质培养。此外，CD34(+)细胞和CD34(+)/CD38(-)细胞（未成熟造血干细胞/祖细胞）的数量也比无基质培养增加得更多。基质细胞产生多种细胞因子，尽管未照射和经X射线照射的细胞与基质细胞的共培养之间差异不大。此外，当经X射线照射的细胞在细胞因子刺激前与基质细胞接触16小时时，观察到类似程度的造血，因此表明细胞间相互作用起关键作用。
本研究结果显示了人间充质干细胞样基质对经照射的造血干细胞/祖细胞进行造血再生的潜在功效。
