Fajardo-Orduña Guadalupe R, Mayani Héctor, Flores-Guzmán Patricia, Flores-Figueroa Eugenia, Hernández-Estévez Erika, Castro-Manrreza Marta, Piña-Sánchez Patricia, Arriaga-Pizano Lourdes, Gómez-Delgado Alejandro, Alarcón-Santos Guadalupe, Balvanera-Ortíz Odette, Montesinos Juan J
Mesenchymal Stem Cells Laboratory, Oncology Research Unit, Oncology Hospital, National Medical Center, IMSS, Mexico City, Mexico.
Hematopoietic Stem Cells Laboratory, Oncology Research Unit, Oncology Hospital, National Medical Center, IMSS, Mexico City, Mexico.
Stem Cells Int. 2017;2017:6061729. doi: 10.1155/2017/6061729. Epub 2017 Nov 9.
Mesenchymal stem/stromal cells (MSCs) from bone marrow (BM) have been used in coculture systems as a feeder layer for promoting the expansion of hematopoietic progenitor cells (HPCs) for hematopoietic cell transplantation. Because BM has some drawbacks, umbilical cord blood (UCB) and placenta (PL) have been proposed as possible alternative sources of MSCs. However, MSCs from UCB and PL sources have not been compared to determine which of these cell populations has the best capacity of promoting hematopoietic expansion. In this study, MSCs from UCB and PL were cultured under the same conditions to compare their capacities to support the expansion of HPCs in vitro. MSCs were cocultured with CD34CD38Lin HPCs in the presence or absence of early acting cytokines. HPC expansion was analyzed through quantification of colony-forming cells (CFCs), long-term culture-initiating cells (LTC-ICs), and CD34CD38Lin cells. MSCs from UCB and PL have similar capacities to increase HPC expansion, and this capacity is similar to that presented by BM-MSCs. Here, we are the first to determine that MSCs from UCB and PL have similar capacities to promote HPC expansion; however, PL is a better alternative source because MSCs can be obtained from a higher proportion of samples.
来自骨髓（BM）的间充质干/基质细胞（MSCs）已被用于共培养系统中作为饲养层，以促进造血祖细胞（HPCs）的扩增，用于造血细胞移植。由于骨髓存在一些缺点，脐带血（UCB）和胎盘（PL）已被提议作为间充质干细胞的可能替代来源。然而，尚未对来自脐带血和胎盘来源的间充质干细胞进行比较，以确定这些细胞群体中哪一个具有促进造血扩增的最佳能力。在本研究中，将来自脐带血和胎盘的间充质干细胞在相同条件下培养，以比较它们在体外支持造血祖细胞扩增的能力。在有或没有早期作用细胞因子的情况下，将间充质干细胞与CD34⁺CD38⁻Lin⁻造血祖细胞共培养。通过定量集落形成细胞（CFCs）、长期培养起始细胞（LTC-ICs）和CD34⁺CD38⁻Lin⁻细胞来分析造血祖细胞的扩增情况。来自脐带血和胎盘的间充质干细胞具有相似的增加造血祖细胞扩增的能力，并且这种能力与骨髓间充质干细胞所呈现的能力相似。在此，我们首次确定来自脐带血和胎盘的间充质干细胞具有相似的促进造血祖细胞扩增的能力；然而，胎盘是更好的替代来源，因为可以从更高比例的样本中获得间充质干细胞。
