El-On J, Ozer L, Gopas J, Sneir R, Golan-Goldhirsh A
The Shraga Segal Department of Microbiology and Immunology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Israel; Laboratory of Parasitology, Soroka University Medical Center, Beer Sheva, Israel.
Phytomedicine. 2009 Aug;16(8):788-92. doi: 10.1016/j.phymed.2009.01.011. Epub 2009 Mar 20.
Several anti-leishmanial drugs of choice are of plant origin. Many of the available drugs against the disease are toxic and in certain cases parasite drug resistance is developed. The development of new compounds is urgently required.
To determine the leishmanicidal activity of the Nuphar lutea plant extract against Leishmania major in vitro.
The leishmanicidal activity of methanolic plant extract against L. major free living promastigotes and intracellular amastigotes was evaluated, using microscopic examinations and the enzymatic XTT assay.
Methanolic extract of N. lutea was highly effective against both Leishmania promastigotes and L. amastigotes (IC(50)=2+/-0.12 microg/ml; ID(50)=0.65+/-0.02 3 microg/ml; LD(50)=2.1+/-0.096 microg/ml, STI=3.23). The extract at 1.25 microg/ml totally eliminated the intracellular parasites within 3 days of treatment. Also, a synergistic anti-leishmanial activity was demonstrated with N. lutea extract combined with the anti-leishmanial drug--paromomycin. The partially purified N. lutea active component was found to be a thermo-stable alkaloid(s) with no electrical charge and is resistant to boiling and to methanol, dichloromethane and xylene treatment.
The present study suggests that N. lutea might be a potential source of anti-leishmanial compounds.
几种治疗利什曼病的首选药物源自植物。许多现有的抗该疾病药物有毒，并且在某些情况下会出现寄生虫耐药性。迫切需要开发新的化合物。
在体外确定黄睡莲植物提取物对硕大利什曼原虫的杀利什曼活性。
使用显微镜检查和酶促XTT测定法评估植物甲醇提取物对硕大利什曼原虫游离前鞭毛体和细胞内无鞭毛体的杀利什曼活性。
黄睡莲甲醇提取物对利什曼原虫前鞭毛体和无鞭毛体均高度有效（IC(50)=2±0.12微克/毫升；ID(50)=0.65±0.023微克/毫升；LD(50)=2.1±0.096微克/毫升，STI=3.23）。1.25微克/毫升的提取物在处理3天内完全消除了细胞内寄生虫。此外，黄睡莲提取物与抗利什曼药物巴龙霉素联合显示出协同抗利什曼活性。发现部分纯化的黄睡莲活性成分是一种不带电荷的热稳定生物碱，对煮沸以及甲醇、二氯甲烷和二甲苯处理具有抗性。
本研究表明黄睡莲可能是抗利什曼化合物的潜在来源。
