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巴拿马利什曼原虫（维安尼亚亚属）：一种利用绿色荧光蛋白表达进行抗利什曼原虫药物筛选的体外试验。

Leishmania (Viannia) panamensis: an in vitro assay using the expression of GFP for screening of antileishmanial drug.

作者信息

Varela M Rubén E, Muñoz Diana Lorena, Robledo Sara M, Kolli Bala K, Dutta Sujoy, Chang Kwang Poo, Muskus Carlos

机构信息

Programa de Estudio y Control de Enfermedades Tropicales-PECET, Universidad de Antioquia, Calle 62 No 52-59, Lab. 632, Medellín, Colombia.

出版信息

Exp Parasitol. 2009 Jun;122(2):134-9. doi: 10.1016/j.exppara.2009.02.012. Epub 2009 Mar 20.

DOI:10.1016/j.exppara.2009.02.012
PMID:19303871
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3640980/
Abstract

Promastigotes of Leishmania (Viannia) panamensis were successfully transfected with p6.5-egfp to express green fluorescent protein. The transfectants remained infective to macrophages, providing an in vitro model for screening antileishmanial drugs. This was demonstrated by flow cytometry of macrophage-associated GFP after exposure of infected cultures to known antileishmanial drugs, i.e. amphotericin B and glucantime. Fluorescence of GFP diminished progressively from infected cells with increasing drug concentrations used in both cases. The availability of this fluorescent assay for infection of macrophages by L. (V.) panamensis facilitates drug discovery program for the Viannia species, which differ significantly from those of the Leishmania subgenus.

摘要

将巴拿马利什曼原虫（维安尼亚亚属）的前鞭毛体用p6.5-egfp成功转染以表达绿色荧光蛋白。转染子对巨噬细胞仍具感染性，为筛选抗利什曼原虫药物提供了一个体外模型。在将感染的培养物暴露于已知的抗利什曼原虫药物（即两性霉素B和葡糖胺锑钠）后，通过对与巨噬细胞相关的绿色荧光蛋白进行流式细胞术证明了这一点。在这两种情况下，随着所用药物浓度的增加，感染细胞中绿色荧光蛋白的荧光逐渐减弱。这种用于巴拿马利什曼原虫（维安尼亚亚属）感染巨噬细胞的荧光测定法的可用性促进了针对维安尼亚亚属物种的药物发现计划，该亚属与利什曼原虫亚属的物种有显著差异。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f33/3640980/8d30d4167179/nihms463079f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f33/3640980/6f95c006c4d7/nihms463079f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f33/3640980/ada47a973df5/nihms463079f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f33/3640980/04b3f5ac2eb8/nihms463079f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f33/3640980/8d30d4167179/nihms463079f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f33/3640980/6f95c006c4d7/nihms463079f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f33/3640980/ada47a973df5/nihms463079f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f33/3640980/04b3f5ac2eb8/nihms463079f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f33/3640980/8d30d4167179/nihms463079f4.jpg

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