[人脱落乳牙来源干细胞的分离与鉴定]
[Isolation and identification of stem cells derived from human exfoliated deciduous teeth].
作者信息
Xu Nuo, Chen Ke, Shen Yuan-Yuan
机构信息
Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Mar;29(3):479-82.
OBJECTIVE
To isolate and identify stem cells from human exfoliated deciduous teeth (SHED).
METHODS
Human pulp tissue were dissected and digested to obtain the single cell suspension. The cell morphology was observed and the clonality of the obtained cells was assessed. The phenotype of the cells was detected by immunohistochemistry and flow cytometry (FCM), and the cell cycle was analyzed. The in vitro differentiation of the cells into adipose tissue and formation of mineralization nodules were evaluated.
RESULTS
Clonogenic assay showed the formation of 16-18 clones in every 10(3) plated cells derived from human exfoliated deciduous teeth. These cells were found to express the markers of mesenchymal stem cells with a multipotent differentiation potential.
CONCLUSION
The cells isolated from human dental pulp are clonogenic and have multipotent differentiation potential, suggesting their identity of SHED.
目的
从人脱落乳牙（SHED）中分离并鉴定干细胞。
方法
解剖并消化人牙髓组织以获得单细胞悬液。观察细胞形态并评估所获细胞的克隆性。通过免疫组织化学和流式细胞术（FCM）检测细胞表型，并分析细胞周期。评估细胞在体外向脂肪组织的分化及矿化结节的形成。
结果
克隆形成试验显示，每10³个接种的来自人脱落乳牙的细胞可形成16 - 18个克隆。发现这些细胞表达具有多能分化潜能的间充质干细胞标志物。
结论
从人牙髓分离出的细胞具有克隆性且有多能分化潜能，表明它们是人脱落乳牙干细胞。
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