Ding Xiang-long, Chen Ke, Shen Yuan-yuan
Department of Stomatdogy Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2011 May;31(5):849-53.
To isolate stem cells from human exfoliated deciduous teeth (SHEDs) and identify their phenotypes and multi-lineage differentiation potential.
Human pulp tissue from exfoliated deciduous teeth were dissected and digested to obtain the single cell suspension. The SHEDs selected by magnetic activated cell sorting system (MACS) were identified by examination of the cell morphology and growth in vitro and detection of the expressions of the cell markers. Osteogenic and adipogenic induction was performed to test the multi-lineage differentiation potential of the cells.
SHEDs were successfully isolated from human exfoliated deciduous teeth. SHEDs showed a lower growth rate than dental pulp cells and displayed high expressions of CD29 and CD105 but low expressions of CD34 and CD45 as shown by flow cytometry. Experiments of in vitro induction demonstrated a strong potential of the STRO-1+ SHEDs for osteogenic and adipogenic differentiation.
Immunomagnetic bead selection can be used to isolate and purify SHEDs, and the STRO-1+ SHEDs show the characteristics of stem cells with multipotent differentiation potentials.
从人脱落乳牙中分离干细胞（SHEDs），并鉴定其表型和多向分化潜能。
解剖并消化人脱落乳牙的牙髓组织以获得单细胞悬液。通过磁性激活细胞分选系统（MACS）筛选出的SHEDs，经细胞形态学检查、体外生长情况及细胞标志物表达检测进行鉴定。进行成骨和成脂诱导以检测细胞的多向分化潜能。
成功从人脱落乳牙中分离出SHEDs。流式细胞术显示，SHEDs的生长速率低于牙髓细胞，高表达CD29和CD105，低表达CD34和CD45。体外诱导实验表明，STRO-1+ SHEDs具有很强的成骨和成脂分化潜能。
免疫磁珠分选法可用于分离和纯化SHEDs，且STRO-1+ SHEDs具有多能分化潜能干细胞的特性。
