麻疯树种子对（60）Co-γ辐射的敏感性及其在辐射育种中的半致死剂量
[Sensitivity of Jatropha curcas seeds to (60)Co-gamma radiation and their medial lethal doses in radiation breeding].
作者信息
Wang Zhao-Yu, Lin Jing-Ming, Luo Li, Xu Zeng-Fu
机构信息
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Mar;29(3):506-8.
OBJECTIVE
To study the sensitivity of Jatropha curcas seeds from three different locations to (60)Co-gamma radiation and to determine the medial lethal doses (LD50) of (60)Co-gamma radiation for these seeds.
METHODS
Six different radiation doses (0, 100, 150, 200, 250 and 300 Gy) were used. Based on the germination rate 50%, LD50 doses of (60)Co-gamma radiation for the seeds were calculated using linear regression equation.
RESULTS
LD50 doses of (60)Co-gamma radiation for these seeds were 178 Gy (seeds from Guangdong), 132 Gy (seeds from Hainan) and 198 Gy (seeds from India) respectively. Increasing radiation doses caused more significant changes in leaf shape of the M1 seedlings.
CONCLUSION
The results provides an important experimental basis for the radiation breeding of the important herbal and energy plant J. curcas.
目的
研究来自三个不同地点的麻疯树种子对（60）Co-γ辐射的敏感性，并确定（60）Co-γ辐射对这些种子的半致死剂量（LD50）。
方法
使用六种不同的辐射剂量（0、100、150、200、250和300 Gy）。根据50%的发芽率，利用线性回归方程计算种子的（60）Co-γ辐射LD50剂量。
结果
这些种子的（60）Co-γ辐射LD50剂量分别为178 Gy（来自广东的种子）、132 Gy（来自海南的种子）和198 Gy（来自印度的种子）。辐射剂量增加导致M1代幼苗的叶片形状发生更显著变化。
结论
该结果为重要草本和能源植物麻疯树的辐射育种提供了重要的实验依据。
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