Shen Ning, Gan Xiao-Liang, Pang Hu-Yu, He Zi-Qing
Department of Anesthesiology, Third Affiliated Hospital of Sun Yat-Sen Uviversity, Guangzhou, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Mar;29(3):528-30.
To investigate the role of sodium cromoglycate in brain protection and its effects on brain tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) expressions after global cerebral ischemia-reperfusion (IR) injury in gerbils.
Twenty-four healthy male gerbils were randomized into 3 equal groups, namely the sham-operated group with isolation of the bilateral carotid arteries but without occlusion, IR injury model group with bilateral carotid artery occlusion, and sodium cromoglycate treatment group with bilateral carotid artery occlusion and sodium cromoglycate administration at 25 mg/kg via the lingual vein as soon as the reperfusion start with another dose 1 h later. The animals were then sacrificed and the thalamus were removed, fixed in 10% formaldehyde and sliced for observation under light microscope with HE staining. The rest brain tissues were prepared into homogenate to determine the content of TNF-alpha and IL-1beta. The right hemispheres of the gerbils were measured for wet weight and dry weight to calculate the water content in the brain.
The water content in the brain of the gerbils in the model group was the highest among the groups, and that in sodium cromoglycate treatment group was significantly less than that of the model group (P<0.05). Microscopic examination showed the most severe brain tissue damage in the model group with also the highest TNF-alpha and IL-1beta levels in the brain. The brain TNF-alpha and IL-1beta levels in sodium cromoglycate group were significantly decreased as compared with those in the model group (P<0.05).
Sodium cromoglycate can alleviate brain IR injury possibly by lowering the TNF-alpha and IL-1beta levels in the brain tissues.
探讨色甘酸钠在沙土鼠全脑缺血再灌注（IR）损伤后脑保护中的作用及其对脑肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）表达的影响。
将24只健康雄性沙土鼠随机分为3组，每组8只。假手术组仅分离双侧颈动脉但不阻断血流；IR损伤模型组双侧颈动脉阻断；色甘酸钠治疗组在双侧颈动脉阻断后，于再灌注开始时经舌静脉给予色甘酸钠25mg/kg，1小时后再给予相同剂量。然后处死动物，取出丘脑，用10%甲醛固定，常规石蜡包埋切片，HE染色，光镜下观察。其余脑组织制成匀浆，测定TNF-α和IL-1β含量。测量沙土鼠右半球湿重和干重，计算脑含水量。
模型组沙土鼠脑含水量最高，色甘酸钠治疗组脑含水量显著低于模型组（P<0.05）。光镜下观察发现，模型组脑组织损伤最严重，脑内TNF-α和IL-1β水平也最高。色甘酸钠组脑内TNF-α和IL-1β水平明显低于模型组（P<0.05）。
色甘酸钠可能通过降低脑组织中TNF-α和IL-1β水平来减轻脑IR损伤。
