Ma Ya-Mei, Wu Bao-Ping, Xia Ou-Dong
Institute of Digestive Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Mar;29(3):541-3.
To detect the mRNA and protein expression of interferon-inducible transmembrane protein-1 (IFITM1) in Peutz-Jeghers syndrome (PJS) and investigate the role of IFITM1 in the occurrence, development and carcinogenesis of PJS polyps.
Reverse transcription-PCR was employed to detect the mRNA expression of IFITM1 in 16 PJS polyp samples, adenomatous polyp tissues, colon adenocarcinoma samples, and normal intestinal mucosal tissues. The protein expression and localization of IFITM1 in these tissues (32 cases for each) were detected with immunohistochemical (IHC) staining.
The IFITM1 mRNA expression was detected in all these tissues, and the expression intensity increased in the order of normal intestinal mucosa, PJS polyp, adenomatous polyp, and colon adenocarcinoma (F=92.704, P=0.000). IHC revealed that IFITM1 protein was localized mainly on the cell membrane and in the cytoplasm, with increased expression intensity in the same order as its mRNA and showing significant differences between the tissues by several rank-sum test (Kruskal-Wallis H, chi(2)=37.036, p=0.000).
The expression level of IFITM1 is associated with the progression of the carcinogenetic process in PJS polyp, and can be used as a sensitive biomarker for diagnosis and prognostic evaluation of PJS.
检测干扰素诱导跨膜蛋白-1（IFITM1）在黑斑息肉综合征（PJS）中的mRNA和蛋白表达，并探讨IFITM1在PJS息肉发生、发展及癌变过程中的作用。
采用逆转录聚合酶链反应（RT-PCR）检测16例PJS息肉样本、腺瘤性息肉组织、结肠腺癌样本及正常肠黏膜组织中IFITM1的mRNA表达。采用免疫组织化学（IHC）染色检测这些组织（各32例）中IFITM1的蛋白表达及定位。
所有这些组织中均检测到IFITM1 mRNA表达，其表达强度按正常肠黏膜、PJS息肉、腺瘤性息肉及结肠腺癌的顺序递增（F=92.704，P=0.000）。免疫组化显示，IFITM1蛋白主要定位于细胞膜和细胞质，其表达强度与mRNA表达强度呈相同顺序增加，经多个秩和检验（Kruskal-Wallis H，χ2=37.036，p=0.000）显示组织间差异有统计学意义。
IFITM1的表达水平与PJS息肉癌变进程相关，可作为PJS诊断及预后评估的敏感生物标志物。
