牛角瓜提取物对四氯化碳诱导的大鼠肝损伤的保肝作用。

Hepatoprotective effects of Calotropis gigantea extract against carbon tetrachloride induced liver injury in rats.

作者信息

Lodhi Gaurav, Singh Hemant Kumar, Pant Kamlesh K, Hussain Zeashan

机构信息

Department of Pharmacology and Therapeutics, Chatrapati Sahuji Maharaj Medical University, Lucknow-226003, India.

出版信息

Acta Pharm. 2009 Mar;59(1):89-96. doi: 10.2478/v10007-009-0002-2.

DOI:10.2478/v10007-009-0002-2

PMID:19304561

Abstract

Ethanolic extract (50 %) of stems of Calotropis gigantea R. Br. (Asclepiadaceae) at doses of 250 and 500 mg kg-1 were studied for hepatoprotective activity in male Wistar rats with liver damage induced using carbon tetrachloride, 2 mL kg-1 twice a week. The protective effect of C. gigantea extract was compared with the standard drug silymarin. Various biochemical parameters such as aspartate amino transferase (AST), alanine amino transferase (ALT), glutathione (GSH), lipid peroxide (LPO), superoxidedismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were evaluated. The results revealed that the C. gigantea extract significantly decreased AST, ALT (p < 0.001) and lipid peroxide (p < 0.01) levels. The antioxidant parameters GSH, GPx, SOD and catalase levels were increased considerably compared to their levels in groups not treated with C. gigantea extract.

摘要

对夹竹桃科植物牛角瓜（Calotropis gigantea R. Br.）茎的乙醇提取物（50%）进行了研究，以250和500 mg kg-1的剂量对用四氯化碳诱导肝损伤的雄性Wistar大鼠进行肝保护活性实验，每周两次，每次2 mL kg-1。将牛角瓜提取物的保护作用与标准药物水飞蓟素进行比较。评估了各种生化参数，如天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、谷胱甘肽（GSH）、脂质过氧化物（LPO）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT）。结果显示，牛角瓜提取物显著降低了AST、ALT（p < 0.001）和脂质过氧化物（p < 0.01）水平。与未用牛角瓜提取物处理的组相比，抗氧化参数GSH、GPx、SOD和过氧化氢酶水平显著升高。