Rathod Nanu R, Chitme Havagiray R, Irchhaiya Raghuveer, Chandra Ramesh
Oman Med J. 2011 Mar;26(2):104-8. doi: 10.5001/omj.2011.26.
To evaluate the hypoglycemic and anti-diabetic activity of chloroform extract of Calotropis gigantea leaves and flowers in normal rats and streptozotocin induced diabetes.
The hypoglycemic activity in normal rats was carried out by treatment using chloroform extract of Calotropis gigantea leaf and flower 10, 20 and 50 mg/kg, orally. The oral glucose tolerance test was carried out by administering glucose (2 g/kg, p.o), to non-diabetic rats treated with leaf and flowers extracts at oral doses 10, 20 and 50 mg/kg, p.o and glibenclamide 10 mg/kg. The serum glucose was then measured at 0, 1.5, 3 and 5 hr after administration of extracts/drug. Streptozotocin-induced diabetic rats were administered the same doses of leaf and flower extracts, and standard drugs glibenclamide was given to the normal rats or 0.5 ml of 5% Tween-80, for 27 days. The blood sample from all groups collected by retro-orbital puncture on 7, 14, 21 and 27th days after administration of the extracts/drug and used for the estimation of serum glucose levels using the glucose kit.
The Calotropis gigantea leaves and flowers extracts were effective in lowering serum glucose levels in normal rats. Improvement in oral glucose tolerance was also registered by treatment with Calotropis gigantean. The administration of leaf and flower extracts to streptozotocin-induced diabetic rats showed a significant reduction in serum glucose levels.
It is concluded that chloroform extracts of Calotropis gigantea leaves and flowers have significant anti-diabetic activity.
评估牛角瓜叶和花的氯仿提取物对正常大鼠及链脲佐菌素诱导的糖尿病大鼠的降血糖和抗糖尿病活性。
通过口服给予正常大鼠10、20和50mg/kg牛角瓜叶和花的氯仿提取物进行降血糖活性实验。对口服剂量为10、20和50mg/kg的叶和花提取物以及10mg/kg格列本脲处理的非糖尿病大鼠，通过口服给予葡萄糖（2g/kg，腹腔注射）进行口服葡萄糖耐量试验。然后在给予提取物/药物后的0、1.5、3和5小时测量血清葡萄糖。给链脲佐菌素诱导的糖尿病大鼠给予相同剂量的叶和花提取物，给正常大鼠给予标准药物格列本脲或0.5ml 5%吐温-80，持续27天。在给予提取物/药物后的第7、14、21和27天，通过眶后穿刺从所有组采集血样，并使用血糖仪用于估计血清葡萄糖水平。
牛角瓜叶和花提取物可有效降低正常大鼠的血清葡萄糖水平。用牛角瓜处理也使口服葡萄糖耐量得到改善。给链脲佐菌素诱导的糖尿病大鼠给予叶和花提取物后，血清葡萄糖水平显著降低。
得出结论，牛角瓜叶和花的氯仿提取物具有显著的抗糖尿病活性。
