嗜氢亚砷酸氧化酶基因的克隆及原位表达研究
Cloning and in situ expression studies of the Hydrogenobaculum arsenite oxidase genes.
作者信息
Clingenpeel Scott R, D'Imperio Seth, Oduro Harry, Druschel Greg K, McDermott Timothy R
机构信息
Thermal Biology Institute and Department of Land Resources & Environmental Sciences, Montana State University, Bozeman, MT 59717, USA.
出版信息
Appl Environ Microbiol. 2009 May;75(10):3362-5. doi: 10.1128/AEM.00336-09. Epub 2009 Mar 20.
Abstract
Novel arsenite [As(III)] oxidase structural genes (aoxAB) were cloned from Hydrogenobaculum bacteria isolated from an acidic geothermal spring. Reverse transcriptase PCR demonstrated expression throughout the outflow channel, and the aoxB cDNA clones exhibited distribution patterns relative to the physicochemical gradients in the spring. Microelectrode analyses provided evidence of quantitative As(III) transformation within the microbial mat.
摘要
从酸性地热泉中分离出的嗜氢菌属细菌中克隆出了新型亚砷酸盐[As(III)]氧化酶结构基因（aoxAB）。逆转录聚合酶链反应表明该基因在整个流出通道中均有表达，并且aoxB cDNA克隆呈现出与泉水中物理化学梯度相关的分布模式。微电极分析提供了微生物垫内As(III)定量转化的证据。
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