一些根结线虫属物种的分子多态性和系统发育关系：在根结线虫属分类学中的应用
Molecular polymorphism and phylogenetic relationship in some Meloidogyne spp.: application to the taxonomy of Meloidogyne.
作者信息
Dalmasso A, Berge J B
机构信息
Institut National de la Recherche Agronomique, Stationde Recherches sur les Nematodes, Antibes, France.
出版信息
J Nematol. 1978 Oct;10(4):323-32.
Proteins and various isozymes were investigated by direct analysis of single specimens in order to check molecular genetic variability, which is not rare in Meloidogyne species in spite of parthenogenetic reproduction. Variability was found in esterases, ocglycerophosphate, malate dehydrogenases, and some other proteins. Other loci appear monomorphic in the genus (for example, catalase), Distinct pools of genes are in a relative accordance with the common described species. Characteristic electrophoretograms are given for M. arenaria, M. javanica, M. incognita, M. hapla, and M. naasi, and it appears that nonspecific esterases are a useful tool supplementing morphology for specific characterization. Because the biochemical evidence is less subjective than the morphological, we believe it is more reliable.
通过对单个样本进行直接分析来研究蛋白质和各种同工酶，以检查分子遗传变异性。尽管根结线虫进行孤雌生殖，但这种变异性在根结线虫物种中并不罕见。在酯酶、α-甘油磷酸酯酶、苹果酸脱氢酶和其他一些蛋白质中发现了变异性。该属中的其他位点似乎是单态的（例如过氧化氢酶）。不同的基因库与常见描述的物种相对一致。给出了南方根结线虫、爪哇根结线虫、花生根结线虫、北方根结线虫和纳西根结线虫的特征电泳图谱，并且似乎非特异性酯酶是补充形态学进行物种特异性鉴定的有用工具。由于生化证据比形态学证据主观性更弱，我们认为它更可靠。
Zotero 插件