Venkatachari S, Payan L A, Dickson D W, Hewlett T E
J Nematol. 1991 Oct;23(4):457-61.
Meloidogyne incognita race 1, M. javanica, M. arenaria race 1, M. hapla, and an undescribed Meloidogyne sp. were analyzed by comparing isozyme phenotypes of esterase, malate dehydrogenase, phosphoglucomutase, isocitrate dehydrogenase, and alpha-glycerophosphate dehydrogenase. Isozyme phenotypes were obtained from single mature females by isoelectric focusing electrophoresis. Of these five isozymes, only esterase and phosphoglucomutase could be used to separate all five Meloidogyne spp.; however, the single esterase electromorphs were similar for M. incognita and M. hapla. Yet when both nematodes were run on the same gel, differences in their esterase phenotypes were detectable. Isozyme phenotypes from the other three isozymes revealed a great deal of similarity among M. incognita, M. javanica, M. arenaria, and the undescribed Meloidogyne sp.
通过比较酯酶、苹果酸脱氢酶、磷酸葡萄糖变位酶、异柠檬酸脱氢酶和α-甘油磷酸脱氢酶的同工酶表型，对南方根结线虫1号小种、爪哇根结线虫、花生根结线虫1号小种、北方根结线虫以及一种未描述的根结线虫进行了分析。通过等电聚焦电泳从单个成熟雌虫中获得同工酶表型。在这5种同工酶中，只有酯酶和磷酸葡萄糖变位酶可用于区分所有5种根结线虫；然而，南方根结线虫和北方根结线虫的单个酯酶电形态相似。不过，当将这两种线虫在同一块凝胶上进行分析时，它们酯酶表型的差异是可检测到的。其他三种同工酶的同工酶表型显示，南方根结线虫、爪哇根结线虫、花生根结线虫以及未描述的根结线虫之间存在很大的相似性。
