Li Tianda, Liu Chousheng, Wang Zhigang, Zhang Liping, Sun Xiuzhu, Zhao Junjin, Meng Fei, Luo Guihe, Zhu Jinqing
College of Animal Science & Technology, Gansu Agricultural University, Lanzhou 730050, China.
Sheng Wu Gong Cheng Xue Bao. 2008 Dec;24(12):2056-60.
Taking Matou goat ear margin as the study material, we succeeded in established a fibroblast cell line by the method of explant culture directly. Observations on morphology, dynamic growth, determination of viability, analysis of karyotype, test of microorganism and other characteristics were detected. Results showed: Population Doubling Time (PDT) of cells was approximately 36 h; Cell viability was 96.7% after thawing; The status of cell After passage was constant; Analysis of chromosomal karyotyps indicated that diploid (2n=60) account for 98% in the cell line. Every index in the cell line met all the standard quality controls of ATCC in USA. The established of Matou goat ear fibroblast cell line has not only important genetic resources preserved at the cell level, but also valuable material for genome, postgenome and somatic cell nuclear transfer research.
以马头山羊耳缘为研究材料，我们通过直接外植体培养法成功建立了成纤维细胞系。对其形态、动态生长、活力测定、核型分析、微生物检测等特性进行了观察。结果表明：细胞群体倍增时间（PDT）约为36小时；解冻后细胞活力为96.7%；传代后细胞状态稳定；染色体核型分析表明，该细胞系中二倍体（2n = 60）占98%。该细胞系各项指标均符合美国ATCC的所有标准质量控制要求。马头山羊耳成纤维细胞系的建立不仅在细胞水平上保存了重要的遗传资源，也是基因组、后基因组和体细胞核移植研究的宝贵材料。
