Shandong Agricultural University, Daizong Road No. 61, Taian 271018, Shandong, People's Republic of China.
Biotechnol Lett. 2012 Mar;34(3):433-40. doi: 10.1007/s10529-011-0799-x. Epub 2011 Nov 22.
A new line of outer root sheath (ORS) cells was established from hair follicles of Jining grey goat by using a mechanical separation combined with enzyme digestion. Cell morphology is described at different phases. The chromosome analysis of ORS cells, identification of the ORS cells and morphological reversion test were detected at the 4th and 40th passages. The ORS cells were healthy and the growth characteristics were stable with a population doubling time of 52 h. Chromosome analysis showed that >58% of cells were diploid. Test for ORS cell line CK19 expression was positive. This newly established ORS cell line not only lays the foundation for further studying on the growth, regeneration, development law of goat hair follicle but also provides a mirror for the research of human hair in medical field.
通过机械分离结合酶消化的方法，从小济宁灰山羊毛囊中建立了一条新的外根鞘（ORS）细胞系。描述了不同阶段的细胞形态。在第 4 代和第 40 代时，对 ORS 细胞的染色体分析、ORS 细胞的鉴定和形态反转试验进行了检测。ORS 细胞健康，倍增时间为 52 小时，生长特征稳定，群体倍增时间为 52 小时。染色体分析显示，>58%的细胞为二倍体。ORS 细胞系 CK19 表达试验阳性。这个新建立的 ORS 细胞系不仅为进一步研究山羊毛囊的生长、再生、发育规律奠定了基础，也为医学领域人类头发的研究提供了一面镜子。
