Aly Hamdy A A, Domènech Oscar, Abdel-Naim Ashraf B
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Al-Azhar University, Nasr City, Cairo, Egypt.
Food Chem Toxicol. 2009 Aug;47(8):1733-8. doi: 10.1016/j.fct.2009.03.019. Epub 2009 Mar 21.
Aroclor 1254 (A1254) has been shown to have potential testicular toxicity. The mechanism of action of A1254 on male reproduction is not clear. The present study was designed to investigate the potential toxicity of A1254 on rat spermatogenesis. Oxidative stress was also assessed in testicular mitochondria as an underlying mechanism. Adult male Wistar rats were injected with A1254 (0, 0.75, 1.5 or 3mg/kg/day i.p.) or with vehicle (corn oil) for 20 consecutive days. A1254 at doses of 1.5 and 3mg/kg/day resulted in a significant decrease in body weight, testes weight, epididymal and relative epididymal weight. Similarly, the relative testis weight was significantly decreased at 3mg/kg/day. Sperm count, motility and daily sperm production were significantly decreased at 1.5 and 3mg/kg/day. The same two doses significantly inhibited the activities of testicular mitochondrial CAT, GPx and GR while the activity of SOD was significantly decreased by 0.75, 1.5 and 3mg/kg/day. The levels of H(2)O(2) generation and LPO were significantly increased in mitochondria in a dose-related pattern. GSH and Vit C were significantly decreased at 0.75, 1.5 and 3mg/kg/day. In conclusion, A1254 impairs spermatogenesis as evidenced, at least partly, by induction of oxidative stress in testicular mitochondria.
多氯联苯混合物1254（A1254）已被证明具有潜在的睾丸毒性。A1254对雄性生殖的作用机制尚不清楚。本研究旨在探讨A1254对大鼠精子发生的潜在毒性。同时评估了睾丸线粒体中的氧化应激作为潜在机制。成年雄性Wistar大鼠连续20天腹腔注射A1254（0、0.75、1.5或3mg/kg/天）或溶剂（玉米油）。1.5和3mg/kg/天剂量的A1254导致体重、睾丸重量、附睾及相对附睾重量显著下降。同样，3mg/kg/天剂量下相对睾丸重量也显著下降。1.5和3mg/kg/天剂量下精子计数、活力和每日精子生成量显著降低。相同的两个剂量显著抑制睾丸线粒体CAT、GPx和GR的活性，而0.75、1.5和3mg/kg/天剂量下SOD的活性显著降低。线粒体中H(2)O(2)生成和LPO水平呈剂量相关模式显著升高。0.75、1.5和3mg/kg/天剂量下GSH和维生素C显著降低。总之，A1254损害精子发生，至少部分证据是睾丸线粒体中氧化应激的诱导。
