Ye Shuji, Nguyen Khoi Tan, Le Clair Stéphanie V, Chen Zhan
Department of Chemistry, University of Michigan, Ann Arbor, 48109, USA.
J Struct Biol. 2009 Oct;168(1):61-77. doi: 10.1016/j.jsb.2009.03.006. Epub 2009 Mar 21.
Sum frequency generation (SFG) vibrational spectroscopy has been demonstrated to be a powerful technique to study the molecular structures of surfaces and interfaces in different chemical environments. This review summarizes recent SFG studies on hybrid bilayer membranes and substrate-supported lipid monolayers and bilayers, the interaction between peptides/proteins and lipid monolayers/bilayers, and bilayer perturbation induced by peptides/proteins. To demonstrate the ability of SFG to determine the orientations of various secondary structures, studies on the interactions between different peptides/proteins (melittin, G proteins, alamethicin, and tachyplesin I) and lipid bilayers are discussed. Molecular level details revealed by SFG in these studies show that SFG can provide a unique understanding on the interactions between a lipid monolayer/bilayer and peptides/proteins in real time, in situ and without any exogenous labeling.
和频振动光谱已被证明是一种研究不同化学环境中表面和界面分子结构的强大技术。本综述总结了最近关于混合双层膜、底物支撑的脂质单层和双层、肽/蛋白质与脂质单层/双层之间的相互作用以及肽/蛋白质引起的双层扰动的和频振动光谱研究。为了证明和频振动光谱确定各种二级结构取向的能力，讨论了关于不同肽/蛋白质（蜂毒素、G蛋白、阿拉霉素和鲎素I）与脂质双层之间相互作用的研究。和频振动光谱在这些研究中揭示的分子水平细节表明，和频振动光谱可以实时、原位且无需任何外源标记地提供对脂质单层/双层与肽/蛋白质之间相互作用的独特理解。
