Department of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, Michigan 48109.
Biointerphases. 2022 May 6;17(3):031202. doi: 10.1116/6.0001859.
This paper summarizes the early research results on studying proteins and peptides at interfaces using sum frequency generation (SFG) vibrational spectroscopy. SFG studies in the C-H stretching frequency region to examine the protein side-chain behavior and in the amide I frequency region to investigate the orientation and conformation of interfacial peptides/proteins are presented. The early chiral SFG research and SFG isotope labeling studies on interfacial peptides/proteins are also discussed. These early SFG studies demonstrate the feasibility of using SFG to elucidate interfacial molecular structures of peptides and proteins in situ, which built a foundation for later SFG investigations on peptides and proteins at interfaces.
本文总结了使用和频产生（SFG）振动光谱研究界面处蛋白质和肽的早期研究成果。本文介绍了在 C-H 伸缩频率区域研究蛋白质侧链行为以及在酰胺 I 频率区域研究界面肽/蛋白质取向和构象的 SFG 研究。还讨论了早期关于界面肽/蛋白质的手性 SFG 研究和 SFG 同位素标记研究。这些早期的 SFG 研究证明了使用 SFG 原位阐明肽和蛋白质界面分子结构的可行性，为后来对界面处肽和蛋白质的 SFG 研究奠定了基础。
