Tonzetich J, Coil J M, Ng W
Department of Oral Biology, Faculty of Dentistry, University of British Columbia, Vancouver.
J Clin Dent. 1991;2(3):79-82.
The present investigation describes a convenient method for collection and analysis of volatile organic compounds from 25 ml mouth air samples. Tenax-GC trapping devices coated with Teflon are used to adsorb and concentrate volatile organic compounds in mouth air at -20 degrees C, which are then thermally desorbed at 140 degrees C. Gas chromatography (GC) analyses are performed using an aluminum column coated with Teflon and packed with 2% poly-MPE on 80/100 mesh Tenax-GC, and employing a flame ionization detector. This procedure allows for amplification of peak heights and detection of compounds that may otherwise escape direct analysis. Of the six prominent peaks detected, identification based on retention times indicates the presence of methanol, acetaldehyde, ethanol and acetone. Volatiles collected using this procedure can be maintained at -20 degrees C for up to 48 hours before analysis. The compact sample tubes allow the system to be easily portable, particularly suitable for sampling breath of persons with localized oral or systemic diseases at locations away from the laboratory. The superiority of this method is that relatively small samples are required for analysis, unlike previously published methods which are based on collection of large volumes of expired air in plastic bags.
本研究描述了一种从25毫升口腔空气样本中收集和分析挥发性有机化合物的简便方法。使用涂有聚四氟乙烯的Tenax-GC捕集装置在-20℃下吸附和浓缩口腔空气中的挥发性有机化合物，然后在140℃下进行热解吸。气相色谱（GC）分析使用涂有聚四氟乙烯并填充有2%聚甲基苯基醚的80/100目Tenax-GC的铝柱，并采用火焰离子化检测器。该程序允许放大峰高并检测否则可能无法直接分析的化合物。在检测到的六个突出峰中，根据保留时间进行的鉴定表明存在甲醇、乙醛、乙醇和丙酮。使用该程序收集的挥发物在分析前可在-20℃下保存长达48小时。紧凑的样品管使该系统易于携带，特别适合在远离实验室的地点对患有局部口腔或全身性疾病的人员进行呼气采样。该方法的优点是分析所需的样品量相对较小，这与以前基于用塑料袋收集大量呼出空气的方法不同。
