Bermel Wolfgang, Bertini Ivano, Csizmok Veronika, Felli Isabella C, Pierattelli Roberta, Tompa Peter
Bruker BioSpin GmbH, Silberstreifen, 76287 Rheinstetten, Germany.
J Magn Reson. 2009 Jun;198(2):275-81. doi: 10.1016/j.jmr.2009.02.012. Epub 2009 Mar 4.
Here, we present a series of exclusively heteronuclear multidimensional NMR experiments, based on 13C direct detection, which exploit the (1)H polarization as a starting source to increase the signal-to-noise ratio. This contributes to make this spectroscopy more useful and usable. Examples are reported for a suitable system such as securin, an intrinsically disordered protein of 22 kDa.
在此,我们展示了一系列基于¹³C直接检测的纯异核多维核磁共振实验,这些实验利用¹H极化作为起始源来提高信噪比。这有助于使这种光谱学更有用且更具实用性。报道了适用于诸如22 kDa的内在无序蛋白securin这样的合适系统的示例。