Bruker BioSpin GmbH, Silberstreifen, 76287 Rheinstetten, Germany.
J Am Chem Soc. 2009 Oct 28;131(42):15339-45. doi: 10.1021/ja9058525.
After the exploitation of (1)H polarization as a starting source for (13)C direct detection experiments, pulse sequences are designed which exploit the accelerated (1)H longitudinal relaxation to expedite (13)C direct detection experiments. We show here that 2D experiments based on (13)C direct detection on a 0.5 mM water sample of ubiquitin can be recorded in a few minutes and 3D experiments in a few hours. We also show that fast methods like nonuniform sampling can be easily implemented. As overall experimental time has always been a counter indication for the use of (13)C direct detection experiments, this research opens new avenues for the application of (13)C NMR to biological molecules.
在利用（1）H 极化作为（13）C 直接检测实验的起始源之后，设计了利用加速（1）H 纵向弛豫来加速（13）C 直接检测实验的脉冲序列。我们在这里表明，基于对 0.5 mM 水样品中泛素的（13）C 直接检测的 2D 实验可以在几分钟内记录，而 3D 实验可以在几个小时内记录。我们还表明，可以轻松实现快速方法，如非均匀采样。由于总体实验时间一直是使用（13）C 直接检测实验的反指标，因此这项研究为（13）C NMR 在生物分子中的应用开辟了新途径。
