Ventura Montse, Pichini Simona, Ventura Rosa, Leal Sonia, Zuccaro Piergiorgio, Pacifici Roberta, de la Torre Rafael
Grup de Recerca en Bioanàlisis i Serveis Analítics, Programa de Recerca en Neuropsicofarmacologia, Institut Municipal d'Investigació Mèdica-Hospital del Mar, Universitat Autónoma de Barcelona, Barcelona, Spain.
Ther Drug Monit. 2009 Apr;31(2):277-80. doi: 10.1097/FTD.0b013e318198670b.
As the stability of drugs of abuse in oral fluid can affect drug testing results, we evaluated this parameter together with recovery for the principal drugs of abuse in two collection devices typically used to ship oral fluid samples to testing laboratories. Two different samples were prepared using Cozart Drug Detection System and Intercept oral fluid collection devices with 600 ng/mL of 6-monoacetylmorphine (6-MAM) and cocaine and 240 ng/mL of Delta-tetrahydrocannabinol (THC) and 11-nor-9-carboxy-Delta-tetrahydrocannabinol (THCCOOH). Samples were sent at ambient temperature by courier to the participating laboratories (n = 19) the same day of preparation. Samples were analyzed upon reception (about 48-72 hours after shipment). Percent coefficients of variation, calculated using robust mean and robust standard deviation, were around 30% for all analytes, except for THCCOOH in both samples (between 50% and 80%) and THC in 1 sample (50%). Percent coefficients of variation were also high (between 50% and 70%) for morphine and benzoylecgonine, formed after 6-MAM and cocaine spontaneous hydrolysis. On average, 9%-12% 6-MAM was converted to morphine and between 26% and 41% cocaine to benzoylecgonine. Good recoveries were observed for the acid metabolite of THC in both collection devices, whereas THC was always scarcely recovered. Depending on the collection device used, obtained results may confound the interpretation and estimation of blood drug concentration, given an oral fluid drug concentration and subsequent consideration of time elapsing from drug consumption.
由于滥用药物在口腔液中的稳定性会影响药物检测结果,我们在两种通常用于将口腔液样本运送至检测实验室的采集装置中,对滥用主要药物的这一参数以及回收率进行了评估。使用Cozart药物检测系统和Intercept口腔液采集装置,制备了两种不同的样本,分别含有600 ng/mL的6-单乙酰吗啡(6-MAM)和可卡因,以及240 ng/mL的Δ-四氢大麻酚(THC)和11-去甲-9-羧基-Δ-四氢大麻酚(THCCOOH)。样本在制备当天通过快递在常温下寄送至参与研究的实验室(n = 19)。样本在接收时(发货后约48 - 72小时)进行分析。使用稳健均值和稳健标准差计算的变异系数百分比,除了两个样本中的THCCOOH(50%至80%之间)和一个样本中的THC(50%)外,所有分析物的变异系数百分比均在30%左右。吗啡和苯甲酰爱康宁(分别由6-MAM和可卡因自发水解形成)的变异系数百分比也很高(50%至70%之间)。平均而言,9% - 12%的6-MAM转化为吗啡,26%至41%的可卡因转化为苯甲酰爱康宁。两种采集装置中THC酸性代谢物的回收率良好,而THC的回收率一直很低。考虑到口腔液药物浓度以及用药后经过的时间,根据所使用的采集装置不同,所得结果可能会混淆对血药浓度的解释和估计。