Moore C, Vincent M, Rana S, Coulter C, Agrawal A, Soares J
Immunalysis Corporation, Pomona, CA 91767, USA.
Forensic Sci Int. 2006 Dec 20;164(2-3):126-30. doi: 10.1016/j.forsciint.2005.12.011. Epub 2006 Jan 19.
This article details the stability of Delta(9)-tetrahydrocannabinol (THC) in oral fluid during collection, extraction and storage. Oral fluid is being increasingly used as the specimen of choice for the detection of drug use in various applications. Studies to determine the extraction efficiency of THC from the collection buffer and stability under various laboratory storage conditions were carried out. THC was extracted from the collection pad and buffer with an average efficiency over 80% and was stable in Quantisal oral fluid extraction buffer when stored at refrigerated temperatures. Fluorescent lighting caused THC losses of over 50%, however the presence of the pad reduced the loss. In the dark, the loss of THC at room temperature was approximately 20% over 14 days. When stored with the serum separators in place, THC losses were significant. After 3 days, THC concentration was reduced by almost 30%, and after 14 days, 60% of the drug was lost and the losses were not concentration dependent.
本文详细介绍了Δ⁹-四氢大麻酚（THC）在口腔液收集、提取和储存过程中的稳定性。在各种应用中，口腔液越来越多地被用作检测药物使用情况的首选样本。开展了多项研究，以确定从收集缓冲液中提取THC的效率以及在各种实验室储存条件下的稳定性。从收集垫和缓冲液中提取THC的平均效率超过80%，并且在冷藏温度下储存在Quantisal口腔液提取缓冲液中时是稳定的。荧光灯会导致超过50%的THC损失，不过收集垫的存在减少了这种损失。在黑暗环境中，室温下THC在14天内的损失约为20%。当与血清分离器一起存放时，THC的损失显著。3天后，THC浓度降低了近30%，14天后，60%的药物损失，且损失与浓度无关。
