Beck Eric T, Lozano Fuentes Saul, Geske David A, Blair Carol D, Beaty Barry J, Black William C
Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
J Mol Evol. 2009 Apr;68(4):403-13. doi: 10.1007/s00239-009-9216-7. Epub 2009 Mar 24.
Aedes triseriatus mosquitoes transovarially transmit (TOT) La Crosse virus (LACV) to their offspring with minimal damage to infected ovaries. Ae. triseriatus inhibitor of apoptosis 1 (AtIAP1) is a candidate gene conditioning the ability to vertically transmit LACV. AtIAP1 was amplified and sequenced in adult mosquitoes reared from field-collected eggs. Sequence analysis showed that AtIAP1 has much higher levels of genetic diversity than genes found in other mosquitoes. Despite this large amount of diversity, strong purifying selection of polymorphisms located in the Baculovirus inhibitor of apoptosis repeat (BIR) domains and, to a lesser extent, in the 5' untranslated region seems to indicate that these portions of AtIAP1 are the most important. These results indicate that the 5'UTR plays an important role in transcription and translation and that the BIR domains are important functional domains in the protein. Single nucleotide polymorphisms (SNPs) were compared between LACV-positive and -negative mosquitoes to test for associations between segregating sites and the ability to be transovarially infected with LACV. Initial results indicated that five SNPs were associated with TOT of LACV; however, these results were not replicable with larger sample sizes.
三带喙库蚊可经卵传递（TOT）拉克罗斯病毒（LACV）给其后代，且对受感染的卵巢损害极小。三带喙库蚊凋亡抑制因子1（AtIAP1）是一个决定垂直传播LACV能力的候选基因。对从野外采集的卵孵化出的成年蚊子中的AtIAP1进行了扩增和测序。序列分析表明，AtIAP1的遗传多样性水平比其他蚊子中的基因高得多。尽管存在大量的多样性，但对杆状病毒凋亡抑制重复序列（BIR）结构域以及在较小程度上对5'非翻译区中的多态性进行的强烈纯化选择似乎表明AtIAP1的这些部分是最重要的。这些结果表明5'UTR在转录和翻译中起重要作用，并且BIR结构域是该蛋白质中的重要功能结构域。比较了LACV阳性和阴性蚊子之间的单核苷酸多态性（SNP），以测试分离位点与经卵感染LACV能力之间的关联。初步结果表明，五个SNP与LACV的经卵传递有关；然而，这些结果在更大样本量下无法重复。
