拉科罗拉病毒感染对三斑家蚊宿主寻找行为和两种神经递质水平的影响。

Effects of La Crosse virus infection on the host-seeking behavior and levels of two neurotransmitters in Aedes triseriatus.

机构信息

Department of Entomology, Virginia Tech, Blacksburg, VA, USA.

Vector-Borne Disease Section, California Department of Public Health, Richmond, CA, USA.

出版信息

Parasit Vectors. 2019 Aug 9;12(1):397. doi: 10.1186/s13071-019-3658-6.

DOI:10.1186/s13071-019-3658-6

PMID:31399119

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6688251/

Abstract

BACKGROUND

La Crosse virus (LACV) infection has been shown to manipulate the blood-feeding behaviors of its main vector, Aedes triseriatus. Here, we investigated the effects of virus infection on serotonin and dopamine and their potential roles in host-seeking. In mosquitoes, serotonin depletion has been shown to interfere with blood-feeding but not host-seeking. Dopamine depletion does not affect either blood-feeding or host-seeking; elevations of dopamine, however, has been shown to inhibit host-seeking. The purpose of this study was to determine the effects of LACV infection on the host-seeking behavior of and neurotransmitter levels in Ae. triseriatus.

METHODS

Host-seeking behavior was evaluated using a uni-port olfactometer and a membrane feeder assay. Levels of serotonin and dopamine in infected and control mosquito heads were measured using HPLC-ED.

RESULTS

Infection with LACV significantly inhibited the activation and attraction of Ae. triseriatus females to a host. A higher proportion of uninfected Ae. triseriatus females were activated by the presence of a host compared to infected mosquitoes and more uninfected mosquitoes were full responders (95.7%) compared to infected ones (91.1%). However, infection with LACV did not significantly affect the landing, probing, or blood-feeding rates of female mosquitoes. LACV-infected mosquitoes had lower serotonin levels than controls (104.5 vs 138.3 pg/head) while the dopamine levels were not affected by infection status (282.3 vs 237 pg/head).

CONCLUSIONS

Our work suggests that virus-induced reduction of serotonin is related to previously reported blood-feeding alterations in LACV-infected mosquitoes and could lead to enhanced transmission and increased vectorial capacity. In addition, some aspects of host-seeking were inhibited by virus infection.

摘要

背景

拉格朗日病毒（LACV）感染已被证明可以操纵其主要载体埃及伊蚊的吸血行为。在这里，我们研究了病毒感染对血清素和多巴胺的影响，以及它们在宿主寻找中的潜在作用。在蚊子中，血清素耗竭已被证明会干扰吸血行为，但不影响宿主寻找。多巴胺耗竭既不影响吸血行为，也不影响宿主寻找；然而，多巴胺的升高已被证明会抑制宿主寻找。本研究的目的是确定 LACV 感染对埃及伊蚊宿主寻找行为和神经递质水平的影响。

方法

使用单端口嗅觉计和膜饲养测定法评估宿主寻找行为。使用 HPLC-ED 测量感染和对照蚊子头部的血清素和多巴胺水平。

结果

LACV 感染显著抑制了埃及伊蚊雌蚊对宿主的激活和吸引。与感染蚊子相比，未感染的埃及伊蚊雌蚊有更高比例被宿主激活，更多未感染的蚊子为完全反应者（95.7%），而感染的蚊子为 91.1%。然而，感染 LACV 并不显著影响雌蚊的着陆、探查或吸血率。感染 LACV 的蚊子的血清素水平低于对照（104.5 比 138.3pg/head），而多巴胺水平不受感染状态的影响（282.3 比 237pg/head）。