Gigli G, Altomonte F, Bocca B, Colombano M, De Grandi R, Ponte M, Triolo A
I Divisione Medicina Ospedale S. Martino, Genova.
Boll Soc Ital Biol Sper. 1991 Mar;67(3):273-8.
Subclinical elevation of urinary albumin excretion is a good predictor of later clinical proteinuria. A simple, sensitive and rapid immunoturbidimetric method was developed to quantify urinary albumin excretion (URIN-PAK ImmunoMICRO LAB, Miles Italia Spa). In the presence of polyethylene glycol 6000, immunocomplex between human albumin and its specific antibody are rapidly formed (5-50 min, at room temperature). Absorbance reading are mode U 340 nm (Automatic Analyzer RA 1000, Technicon). The test is specific for albumin failing to cross react with other plasma proteins present in urine, as well as with glibenclamide, chlorpropamide, phenformin, hemoglobin, glucose, urea and thymol. The present method correlates with SCLAVO H-ALBUMIN RIA Kit (r = 0.9917). The test is suitable for clinical use.
尿白蛋白排泄的亚临床升高是后期临床蛋白尿的良好预测指标。我们开发了一种简单、灵敏且快速的免疫比浊法来定量尿白蛋白排泄（URIN-PAK ImmunoMICRO LAB，Miles Italia Spa公司）。在聚乙二醇6000存在的情况下，人白蛋白与其特异性抗体之间的免疫复合物会迅速形成（在室温下5至50分钟）。吸光度读数在340 nm处进行（使用Technicon公司的自动分析仪RA 1000）。该检测对白蛋白具有特异性，不会与尿液中存在的其他血浆蛋白以及格列本脲、氯磺丙脲、苯乙双胍、血红蛋白、葡萄糖、尿素和麝香草酚发生交叉反应。本方法与SCLAVO H-ALBUMIN RIA试剂盒相关（r = 0.9917）。该检测适用于临床应用。
