Ojeniyi B, Birch-Andersen A, Mansa B, Rosdahl V T, Høiby N
Staphylococcus Laboratory, Rigshospitalet, Statens Seruminstitut, Copenhagen, Denmark.
APMIS. 1991 Oct;99(10):925-30.
Sixteen phage suspensions isolated from the sputum of sixteen cystic fibrosis patients and five phages from the present phage typing set were studied by electron microscopy. All sputum samples contained at least one type of bacteriophage (range: 1-4) which could be classified by the morphology and dimensions of the virion. All phages isolated from sputum as well as the four typing phages were tailed phages. The clinical phages belonged either to the Myoviridae, Siphoviridae or Podoviridae family. The four typing phages belonged to the Myoviridae family. The detection of the presence of tailed phages in sputum further supports the prevailing theory that phages play a role in the phenotypical change of Pseudomonas aeruginosa during the chronic lung infection of cystic fibrosis patients, since only tailed phages are known to be temporate and thus mediate transduction and conversion.
通过电子显微镜对从16名囊性纤维化患者痰液中分离出的16种噬菌体悬液以及现有噬菌体分型组中的5种噬菌体进行了研究。所有痰液样本均至少含有一种可根据病毒体形态和尺寸分类的噬菌体（范围：1 - 4种）。从痰液中分离出的所有噬菌体以及4种分型噬菌体均为有尾噬菌体。临床分离的噬菌体属于肌尾噬菌体科、长尾噬菌体科或短尾噬菌体科。4种分型噬菌体属于肌尾噬菌体科。痰液中有尾噬菌体的检测进一步支持了目前的理论，即噬菌体在囊性纤维化患者慢性肺部感染期间铜绿假单胞菌的表型变化中发挥作用，因为已知只有有尾噬菌体是温和噬菌体，因此可介导转导和转化。
