Ambroa Antón, Blasco Lucia, López-Causapé Carla, Trastoy Rocio, Fernandez-García Laura, Bleriot Ines, Ponce-Alonso Manuel, Pacios Olga, López Maria, Cantón Rafael, Kidd Timothy J, Bou German, Oliver Antonio, Tomás Maria
Microbiology Department-Research Institute Biomedical A Coruña (INIBIC), Hospital A Coruña (CHUAC), University of A Coruña (UDC), A Coruña, Spain.
Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA), Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), Madrid, Spain.
Front Microbiol. 2020 Sep 25;11:556706. doi: 10.3389/fmicb.2020.556706. eCollection 2020.
Bacteriophages are important in bacterial ecology and evolution. is the most prevalent bacterial pathogen in chronic bronchopulmonary infection in cystic fibrosis (CF). In this study, we used bioinformatics, microbiological and microscopy techniques to analyze the bacteriophages present in 24 isolates belonging to the international CF clone (ST274-CC274). Interestingly, we detected the presence of five members of the family of prophages (Pf1, Pf4, Pf5, Pf6, Pf7), which have previously been observed in . In addition, we identified a new filamentous prophage, designated Pf8, in the AUS411.500 isolate belonging to the international CF clone. We detected only one prophage, never previously described, from the family (with 66 proteins and displaying homology with PHAGE_Pseudo_phi297_NC_016762). This prophage was isolated from the AUS531 isolate carrying a new gene which is implicated in the phage infection ability, named Bacteriophage Control Infection (). We characterized the role of the Bci protein in bacteriophage infection and in regulating the host Quorum Sensing (QS) system, motility and biofilm and pyocyanin production in the isogenic mutant AUS531Δ isolate. The findings may be relevant for the identification of targets in the development of new strategies to control infections, particularly in CF patients.
噬菌体在细菌生态学和进化中具有重要意义。铜绿假单胞菌是囊性纤维化（CF）慢性支气管肺部感染中最常见的细菌病原体。在本研究中，我们运用生物信息学、微生物学和显微镜技术，对属于国际CF克隆（ST274-CC274）的24株铜绿假单胞菌分离株中存在的噬菌体进行了分析。有趣的是，我们检测到了原噬菌体家族的五个成员（Pf1、Pf4、Pf5、Pf6、Pf7），这些成员此前在铜绿假单胞菌中已被观察到。此外，我们在属于国际CF克隆的AUS411.500分离株的铜绿假单胞菌中鉴定出一种新的丝状原噬菌体，命名为Pf8。我们从肌尾噬菌体科中仅检测到一种此前从未描述过的原噬菌体（有66种蛋白质，与噬菌体_Pseudo_phi297_NC_016762具有同源性）。这种原噬菌体是从携带一个与噬菌体感染能力相关的新基因（名为噬菌体控制感染基因，Bci）的AUS531分离株的铜绿假单胞菌中分离出来的。我们对Bci蛋白在噬菌体感染以及调控宿主群体感应（QS）系统、运动性、生物膜形成和绿脓菌素产生方面的作用，在同基因突变体AUS531Δ分离株的铜绿假单胞菌中进行了表征。这些发现可能与确定控制铜绿假单胞菌感染新策略开发中的靶点相关，特别是在CF患者中。
