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3':5'-环磷酸腺苷依赖性蛋白激酶在网织红细胞裂解物蛋白质合成调控中的作用

Role of 3':5'-cyclic-AMP-dependent protein kinase in regulation of protein synthesis in reticulocyte lysates.

作者信息

Datta A, de Haro C, Sierra J M, Ochoa S

出版信息

Proc Natl Acad Sci U S A. 1977 Apr;74(4):1463-7. doi: 10.1073/pnas.74.4.1463.

DOI:10.1073/pnas.74.4.1463
PMID:193101
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC430801/
Abstract

The initiation inhibitor of reticulocyte lysates has been shown by others to be associated with a 3':5'-cyclic-AMP-independent protein kinase that catalyzes the phosphorylation of the small (38,000 daltons) subunit of the polypeptide chain initiation factor eIF-2. This factor forms a ternary complex with Met-tRNAi and GTP which, on interaction with a 40S ribosome, gives rise to a 40S complex. Ternary complex formation is inhibited by prior incubation of partially purified eIF-2 with reticulocyte inhibitor and ATP. The relation between phosphorylation and inactivation of eIF-2 is indicated by the lack of inhibition when ATP is omitted. Translation in hemin-containing reticulocyte lysates is also inhibited by cyclic-AMP-dependent protein kinases or their catalytic subunits. They act by converting proinhibitor (inactive eIF-2 kinase) present in lysates to inhibitor (active eIF-2 kinase). This reaction is analogous to the conversion of inactive phosphorylase kinase to active phosphorylase kinase.

摘要

其他人已证明，网织红细胞裂解物的起始抑制剂与一种不依赖3':5'-环磷酸腺苷的蛋白激酶相关，该激酶催化多肽链起始因子eIF-2的小（38,000道尔顿）亚基的磷酸化。该因子与甲硫氨酰-tRNAi和GTP形成三元复合物，该复合物与40S核糖体相互作用时会产生40S复合物。在将部分纯化的eIF-2与网织红细胞抑制剂和ATP预先孵育后，三元复合物的形成受到抑制。当省略ATP时缺乏抑制作用，这表明了eIF-2磷酸化与失活之间的关系。含血红素的网织红细胞裂解物中的翻译也受到依赖环磷酸腺苷的蛋白激酶或其催化亚基的抑制。它们通过将裂解物中存在的前抑制剂（无活性的eIF-2激酶）转化为抑制剂（活性eIF-2激酶）来发挥作用。该反应类似于无活性的磷酸化酶激酶转化为活性磷酸化酶激酶。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8f3/430801/cf9c278c320c/pnas00026-0170-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8f3/430801/cf9c278c320c/pnas00026-0170-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8f3/430801/cf9c278c320c/pnas00026-0170-a.jpg

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Role of 3':5'-cyclic-AMP-dependent protein kinase in regulation of protein synthesis in reticulocyte lysates.3':5'-环磷酸腺苷依赖性蛋白激酶在网织红细胞裂解物蛋白质合成调控中的作用
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Regulation of protein synthesis.蛋白质合成的调控
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Regulation of protein synthesis in rabbit reticulocyte lysates by the heme-regulated protein kinase: inhibition of interaction of Met-tRNAfMet binding factor with another initiation factor in formation of Met-tRNAfMet.40S ribosomal subunit complexes.血红素调节蛋白激酶对兔网织红细胞裂解物中蛋白质合成的调节：在甲硫氨酰 - 起始转运核糖核酸（Met - tRNAfMet）与40S核糖体亚基复合物形成过程中，抑制Met - tRNAfMet结合因子与另一种起始因子的相互作用。
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引用本文的文献

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Plant Physiol. 1987 Apr;83(4):988-93. doi: 10.1104/pp.83.4.988.
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Mechanism of interferon action: phosphorylation of protein synthesis initiation factor eIF-2 in interferon-treated human cells by a ribosome-associated kinase processing site specificity similar to hemin-regulated rabbit reticulocyte kinase.干扰素作用机制：在经干扰素处理的人细胞中，核糖体相关激酶使蛋白质合成起始因子eIF-2磷酸化，该激酶的加工位点特异性类似于血红素调节的兔网织红细胞激酶。
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Regulation of protein synthesis in rabbit reticulocyte lysates: purification and characterization of heme-reversible translational inhibitor.兔网织红细胞裂解物中蛋白质合成的调控：血红素可逆性翻译抑制剂的纯化与特性分析
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Cell-free hemoglobin synthesis. II. Characteristics of the transfer ribonucleic acid-dependent assay system.无细胞血红蛋白合成。II. 依赖转移核糖核酸的检测系统的特性。
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Control of globin synthesis by hemin: factors influencing formation of an inhibitor of globin chain initiation in reticulocyte lysates.血红素对珠蛋白合成的调控：影响网织红细胞裂解物中珠蛋白链起始抑制剂形成的因素。
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Met-tRNAfMet binding to 40S ribosomal subunits: a site for the regulation of initiation of protein synthesis by hemin.甲硫氨酰 - 起始tRNA与40S核糖体亚基的结合：血红素调节蛋白质合成起始的位点。
Proc Natl Acad Sci U S A. 1974 Aug;71(8):2946-50. doi: 10.1073/pnas.71.8.2946.
8
The effects of hemin and double-stranded RNA on alpha and beta globin synthesis in reticulocyte and Krebs II ascites cell-free systems and the relationship of these effects to an initiation factor preparation.氯化血红素和双链RNA对网织红细胞及克雷布斯II型腹水无细胞系统中α和β珠蛋白合成的影响，以及这些影响与一种起始因子制剂的关系。
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Physical properties of a purified cyclic adenosine 3':5'-monophosphate-dependent protein kinase from bovine heart muscle.从牛心肌中纯化得到的环磷腺苷（cAMP）依赖性蛋白激酶的物理性质
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