甲硫氨酰 - 起始tRNA与40S核糖体亚基的结合：血红素调节蛋白质合成起始的位点。
Met-tRNAfMet binding to 40S ribosomal subunits: a site for the regulation of initiation of protein synthesis by hemin.
作者信息
Clemens M J, Henshaw E C, Rahamimoff H, London I M
出版信息
Proc Natl Acad Sci U S A. 1974 Aug;71(8):2946-50. doi: 10.1073/pnas.71.8.2946.
Abstract
On incubation of reticulocyte lysates at 30 degrees in the absence of added hemin, protein synthesis declines sharply within 4-6 min, due to the action of a translational inhibitor. Partially purified preparations of this inhibitor, in concentrations that inhibit protein synthesis in the lysate, cause reduced binding of Met-tRNA(f) (Met) to derived 40S ribosomal subunits in a ribosomal-salt-wash-dependent assay system. Neither the association of salt wash proteins with the subunits nor the level of Met-tRNA(f) (Met) bound in preformed 40S complexes is reduced by the inhibitor. No Met-tRNA(f) (Met) deacylase activity could be detected in the inhibitor preparation. Protein synthesis in reticulocyte lysates lacking added hemin or containing exogenous inhibitor is maintained by addition of small amounts of an initiation preparation factor, "F-MP," which may be involved in the binding of Met-tRNA(f) (Met) to 40S subunits. This binding constitutes a site for control of protein synthesis by hemin in reticulocytes.
摘要
在无添加血红素的情况下，将网织红细胞裂解物于30摄氏度孵育，由于一种翻译抑制剂的作用，蛋白质合成在4 - 6分钟内急剧下降。这种抑制剂的部分纯化制剂，在能够抑制裂解物中蛋白质合成的浓度下，在核糖体盐洗依赖性测定系统中会导致甲硫氨酰 - tRNA(f)(Met)与衍生的40S核糖体亚基的结合减少。该抑制剂既不降低盐洗蛋白与亚基的结合，也不降低在预先形成的40S复合物中结合的甲硫氨酰 - tRNA(f)(Met)水平。在抑制剂制剂中未检测到甲硫氨酰 - tRNA(f)(Met)脱酰酶活性。通过添加少量起始制备因子“F - MP”可维持缺乏添加血红素或含有外源性抑制剂的网织红细胞裂解物中的蛋白质合成，“F - MP”可能参与甲硫氨酰 - tRNA(f)(Met)与40S亚基的结合。这种结合构成了血红素在网织红细胞中控制蛋白质合成的一个位点。
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Proc Natl Acad Sci U S A. 1974 Aug;71(8):2946-50. doi: 10.1073/pnas.71.8.2946.
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