Chan T S
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1734-8. doi: 10.1073/pnas.74.4.1734.
Herpes simplex virus type 1 induces deoxycytidine deaminase (cytidine/deoxycytidine aminohydrolase, EC 3.5.4.5) activity when it lytically infects a number of mammalian cell lines. The deaminase activity is induced in a mouse cell line that is deficient in this enzyme. The induction of the enzyme in this mutant cell line does not occur in the presence of actinomycin D and the induced enzyme is more thermolabile than the enzyme of the wild-type mouse cell line. Furthermore, a new deoxycytidine deaminase species with a characteristic electrophoretic mobility that is different from that of the host cell enzyme is found in cell extracts prepared from a human cell line infected with herpesvirus. These results strongly suggest that the virus-induced deoxycytidine deaminase is coded by the viral genome. Because a deficiency in this enzyme is conditionally lethal for cells growing in a medium containing 5-methyldeoxycytidine as the sole source of thymidylate, this enzyme can be utilized as a selective marker for selecting mutant cells that have regained deoxycytidine deaminase activity as the result of infection by ultraviolet-inactivated herpes simplex virus.
单纯疱疹病毒1型在裂解感染多种哺乳动物细胞系时会诱导脱氧胞苷脱氨酶（胞苷/脱氧胞苷氨基水解酶，EC 3.5.4.5）的活性。在缺乏这种酶的小鼠细胞系中也会诱导出脱氨酶活性。在这种突变细胞系中，放线菌素D存在时酶的诱导不会发生，且诱导出的酶比野生型小鼠细胞系的酶更不耐热。此外，在感染疱疹病毒的人细胞系制备的细胞提取物中发现了一种具有特征性电泳迁移率的新的脱氧胞苷脱氨酶，其与宿主细胞酶的电泳迁移率不同。这些结果强烈表明，病毒诱导的脱氧胞苷脱氨酶是由病毒基因组编码的。由于这种酶的缺乏对在含有5-甲基脱氧胞苷作为胸苷酸唯一来源的培养基中生长的细胞具有条件致死性，因此这种酶可作为一种选择标记，用于筛选因感染紫外线灭活的单纯疱疹病毒而恢复脱氧胞苷脱氨酶活性的突变细胞。
