开发一种免疫结合斑点印迹法作为人类囊尾蚴病血清诊断的替代方法。
Development of an immunobinding dot-blot assay as an alternative for the serodiagnosis of human cysticercosis.
作者信息
Hernández-Cruz E, González-Cabriales J J, Ordaz-Pichardo C, de la Cruz-Hernández N I, Flores-Gutiérrez G H
机构信息
Unidad Académica Multidisciplinaria Reynosa-Aztlán, Universidad Autónoma de Tamaulipas, Reynosa, Tamaulipas, México.
出版信息
J Helminthol. 2009 Dec;83(4):333-7. doi: 10.1017/S0022149X09270866. Epub 2009 Mar 25.
An immunobinding dot-blot assay (DBA) was developed on nitrocellulose paper for the serodiagnosis of human cysticercosis, using Cysticercus cellulosae as antigen. The DBA had an immunological sensitivity of 0.08 mg of antigen protein/ml; however, it showed cross-reactions with antigens of adult Taenia solium and Echinococcus granulosus, but not with Toxoplasma gondii and Entamoeba histolytica antigens. An enzyme-linked immunosorbent assay (ELISA) was used as the gold standard for obtaining the diagnostic validity of the DBA, giving 84.61%, 100.00%, 100.00% and 97.98% for epidemiological sensitivity, epidemiological specificity and positive and negative predictive values, respectively. There were no statistical differences between the two tests (P < 0.05, kappa = 0.907). This study showed that DBA is an alternative method for the serodiagnosis of human cysticercosis.
采用猪囊尾蚴作为抗原，在硝酸纤维素纸上建立了免疫结合斑点印迹法（DBA）用于人体囊尾蚴病的血清学诊断。DBA的免疫敏感性为0.08 mg抗原蛋白/ml；然而，它与猪带绦虫成虫和细粒棘球绦虫的抗原存在交叉反应，但与弓形虫和溶组织内阿米巴抗原无交叉反应。采用酶联免疫吸附测定法（ELISA）作为金标准来确定DBA的诊断有效性，其流行病学敏感性、流行病学特异性、阳性预测值和阴性预测值分别为84.61%、100.00%、100.00%和97.98%。两种检测方法之间无统计学差异（P < 0.05，kappa = 0.907）。本研究表明，DBA是人体囊尾蚴病血清学诊断的一种替代方法。
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