Rinkevich F D, Scott J G
Department of Entomology, Cornell University, Ithaca, NY 14853-0901, USA.
Insect Mol Biol. 2009 Apr;18(2):233-42. doi: 10.1111/j.1365-2583.2009.00873.x.
Sequence analysis of 168 cDNA clones encoding 12 nicotinic acetylcholine receptor subunits, Tcasalpha1-Tcasalpha11 and Tcasbeta1, from the red flour beetle, Tribolium castaneum, revealed extensive post-transcriptional modification and multiple alleles. The greatest diversity was found for Tcasa6, where 18 unique transcripts, as a result of alternative and optional exon usage, were seen. A novel alternative exon 8d was found in one Tcasalpha6 transcript. Tcasalpha5 transcripts did not contain previously reported exons 8-10. Six subunits had transcripts that contained unspliced introns, which introduced premature stop codons. Intron 3' splice site variants were seen at six intron boundaries across five subunits. A-to-I RNA editing was seen only in Tcasalpha6. Alleles were found for all subunit genes, except Tcasalpha1 and Tcasalpha10. Transcriptional and allelic diversity are discussed with respect to receptor function and potential interactions with insecticides.
对来自赤拟谷盗（Tribolium castaneum）的168个编码12种烟碱型乙酰胆碱受体亚基（Tcasalpha1 - Tcasalpha11和Tcasbeta1）的cDNA克隆进行序列分析，揭示了广泛的转录后修饰和多个等位基因。在Tcasa6中发现了最大的多样性，由于可变外显子和选择性外显子的使用，出现了18种独特的转录本。在一个Tcasalpha6转录本中发现了一个新的可变外显子8d。Tcasalpha5转录本不包含先前报道的外显子8 - 10。六个亚基的转录本包含未剪接的内含子，这些内含子引入了过早的终止密码子。在五个亚基的六个内含子边界处发现了内含子3'剪接位点变体。仅在Tcasalpha6中发现了A到I的RNA编辑。除了Tcasalpha1和Tcasalpha10外，在所有亚基基因中都发现了等位基因。本文讨论了转录和等位基因多样性与受体功能以及与杀虫剂潜在相互作用的关系。
