核糖核酸酶E通过直接结合大肠杆菌rne 5'非翻译区的一个茎环结构来自动调节其自身的合成。
RNase E autoregulates its synthesis in Escherichia coli by binding directly to a stem-loop in the rne 5' untranslated region.
作者信息
Schuck Alyssa, Diwa Alexis, Belasco Joel G
机构信息
Kimmel Center for Biology and Medicine at the Skirball Institute, New York University School of Medicine, New York, NY 10016, USA.
出版信息
Mol Microbiol. 2009 Apr;72(2):470-8. doi: 10.1111/j.1365-2958.2009.06662.x. Epub 2009 Mar 6.
Abstract
RNase E autoregulates its production in Escherichia coli by governing the decay rate of rne (RNase E) mRNA. It does so by a mechanism that is dependent in part on hp2, a cis-acting stem-loop within the rne 5' untranslated region. In principle, hp2 could function either as a cleavage site for RNase E or as a binding site for that protein or an ancillary factor. Here we show that the effector region at the top of hp2 is cleaved poorly by RNase E yet binds the catalytic domain of that ribonuclease with a sequence specificity reflecting its efficacy in feedback regulation. These findings suggest that hp2 controls RNase E synthesis by binding to RNase E and expediting cleavage elsewhere within the rne transcript.
摘要
核糖核酸酶E（RNase E）通过控制rne（RNase E）信使核糖核酸的降解速率来自动调节其在大肠杆菌中的产生。它通过一种部分依赖于hp2的机制来实现这一点，hp2是rne 5'非翻译区内的一个顺式作用茎环结构。原则上，hp2既可以作为RNase E的切割位点，也可以作为该蛋白质或辅助因子的结合位点。在这里，我们表明，hp2顶部的效应区域被RNase E切割的效率很低，但能以反映其在反馈调节中的效力的序列特异性结合该核糖核酸酶的催化结构域。这些发现表明，hp2通过与RNase E结合并加速rne转录本其他部位的切割来控制RNase E的合成。
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