USEPA, National Risk Management Research Laboratory, Water Supply/Water Resources Division, Cincinnati, OH 45268, USA.
J Appl Microbiol. 2009 Sep;107(3):964-9. doi: 10.1111/j.1365-2672.2009.04276.x. Epub 2009 Mar 25.
To screen for the virulence potential of Aeromonas isolates based on the change in regulation of c-jun and c-fos in the human intestinal tissue culture cell line Caco-2.
Aeromonas cells were added to Caco-2 cells at a ratio of approx. 1 : 1. After 1-, 2- and 3-h incubation at 37 degrees C, mRNA was extracted from the cells and gene expression of two host genes, c-jun and c-fos, quantified. Aeromonas isolates which were pathogenic in the neonatal mouse model demonstrated up-regulation of c-jun and c-fos compared to avirulent isolates.
Human cell culture results showed that c-jun and c-fos were predictive of Aeromonas virulence.
An Aeromonas relative virulence scale is proposed for use in the testing of Aeromonas drinking water isolates.
基于人肠道组织培养细胞系 Caco-2 中 c-jun 和 c-fos 调控变化，筛选气单胞菌分离株的毒力潜能。
以约 1:1 的比例将气单胞菌细胞添加到 Caco-2 细胞中。在 37°C 孵育 1、2 和 3 小时后，从细胞中提取 mRNA，并定量检测两个宿主基因 c-jun 和 c-fos 的基因表达。与无毒力分离株相比，在新生小鼠模型中具有致病性的气单胞菌分离株显示 c-jun 和 c-fos 的上调。
人体细胞培养结果表明，c-jun 和 c-fos 可预测气单胞菌的毒力。
提出了气单胞菌相对毒力量表，用于测试气单胞菌饮用水分离株。
