Rovira i Virgili University, Department of Basic Medical Sciences, Mycology and Environmental Microbiology Unit, Reus, Spain.
Pere Virgili Health Research Institute (IISPV), Reus, Spain.
Front Immunol. 2022 Jul 8;13:875689. doi: 10.3389/fimmu.2022.875689. eCollection 2022.
are autochthonous bacteria of aquatic environments that are considered to be emerging pathogens to humans, producing diarrhea, bacteremia, and wound infections. Genetic identification shows that 95.4% of the strains associated with clinical cases correspond to the species (37.26%), (23.49%), (21.54%), and (13.07%). However, few studies have investigated the human immune response against some spp. such as , , and . The present study aimed to increase the knowledge about the innate human immune response against six species, using, for the first time, an infection model with the monocytic human cell line THP-1, and to evaluate the intracellular survival, the cell damage, and the expression of 11 immune-related genes (, , , , , , , , , , and ). Transcriptional analysis showed an upregulated expression of a variety of the monocytic immune-related genes, with a variable response depending upon the species. The species that produced the highest cell damage, independently of the strain origin, coincidentally induced a higher expression of immune-related genes and corresponded to the more prevalent clinical species , , and . Additionally, monocytic cells showed an overexpression of the apoptotic and pyroptotic genes involved in cell death after , , and infection. However, the apoptosis route seemed to be the only way of producing cell damage and death in the case of the species and , while apparently only used the pyroptosis route.
是水生环境中的土著细菌，被认为是人类的新兴病原体，可导致腹泻、菌血症和伤口感染。基因鉴定表明，与临床病例相关的菌株中 95.4% 属于物种 （37.26%）、 （23.49%）、 （21.54%）和 （13.07%）。然而，很少有研究调查过人类针对某些 spp. 的免疫反应，例如 、 和 。本研究旨在通过使用单核人细胞系 THP-1 首次建立 感染模型，增加对人类固有免疫反应针对六种 物种的认识，并评估细胞内存活、细胞损伤和 11 种免疫相关基因的表达（、、、、、、、、和 ）。转录分析显示，多种单核细胞免疫相关基因的表达上调，其反应因物种而异。产生最高细胞损伤的物种，与菌株来源无关，巧合的是诱导了更高水平的免疫相关基因表达，并且与更常见的临床物种 、 和 相对应。此外，单核细胞在感染 、 和 后，凋亡和焦亡相关基因的表达过度，涉及细胞死亡。然而，在 和 的情况下，凋亡途径似乎是产生细胞损伤和死亡的唯一途径，而 显然只使用了焦亡途径。
