通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对分枝杆菌属进行鉴定
Characterization of Mycobacterium species by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
作者信息
Fevre A, Fougerat J, Bruneau S, Guinet R
机构信息
Centre d'Immunochimie Microbienne, Institut Pasteur, Lentilly, France.
出版信息
Appl Theor Electrophor. 1991;2(1):13-6.
PMID:1932205
Abstract
A rapid method has been standardized for extraction and SDS-PAGE analyses of mycobacterial cellular protein. Complex patterns were reproducibly obtained of the 31 reference strains or clinical isolates studied, belonging to 11 species. These patterns were species specific; thus this method could be an easy tool for mycobacterial strain characterization.
摘要
已标准化了一种用于分枝杆菌细胞蛋白提取和SDS-PAGE分析的快速方法。在所研究的属于11个种的31个参考菌株或临床分离株中，可重复获得复杂的图谱。这些图谱具有种特异性；因此该方法可能是一种用于分枝杆菌菌株鉴定的简便工具。
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