Qu Zhen-Yu, Yang Cong, Wang Wei-Hong, Xu Biao
Dept. of Oral and Maxillofacial Surgery, College of Stomatology, Kunming Medical University, Kunming 650031, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2009 Feb;27(1):75-8.
To evaluate the feasibility of repairing the long defects in rabbits by double-bridging technique and chitosan-collagen chambers.
Thirty Chinese rabbits were divided into three groups randomly. 15 mm defects in right facial nerve of rabbits were repaired in three groups: double-bridging (group I ), nerve autografts (group II), and normal control (group III). General observation, electrophysiological study, histological study and image analysis were performed 12 weeks postoperatively. All results were used to evaluate the nerve regeneration.
Chitosan-collagen chambers were obviously degraded 12 weeks postoperatively and there was no foreign body reaction at this stage. They also restrain the formation of neuroma and provided a good microcircumambience for nerve regeneration. The recovery of nerve regeneration were good in group I. The nerve conduction velocity (NCV) and compound muscle action potential (CMAP) of regenerated nerve were analyzed, which showed that there was no significant difference between the group I and group II (P > 0.05), but they all did not recover to normal level(P < 0.01).
Double-bridging technique is a simple method for treatment of long nerve defect and its effect is certain. Chitosan-collage chambers is suitable to implant into body in order to repair a nerve defect with excellent biocompatibility.
评估采用双桥技术和壳聚糖-胶原腔室修复兔长节段神经缺损的可行性。
将30只中国白兔随机分为三组。三组均修复兔右侧面神经15 mm缺损：双桥组（Ⅰ组）、自体神经移植组（Ⅱ组）和正常对照组（Ⅲ组）。术后12周进行大体观察、电生理研究、组织学研究和图像分析。所有结果均用于评估神经再生情况。
壳聚糖-胶原腔室在术后12周明显降解，此阶段无异物反应。它们还抑制神经瘤形成，并为神经再生提供良好的微环境。Ⅰ组神经再生恢复良好。分析再生神经的神经传导速度（NCV）和复合肌肉动作电位（CMAP），结果显示Ⅰ组与Ⅱ组之间无显著差异（P>0.05），但均未恢复至正常水平（P<0.01）。
双桥技术是治疗长节段神经缺损的一种简便方法，效果肯定。壳聚糖-胶原腔室具有良好的生物相容性，适合植入体内修复神经缺损。
