Guo Bao-Feng, Dong Ming-Min
Department of Otolaryngology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Otolaryngol Head Neck Surg. 2009 Feb;140(2):159-64. doi: 10.1016/j.otohns.2008.10.039.
To observe the curative effect of neural stem cells (NSCs), which are used in tissue-engineered artificial nerve, on repairing rabbit 10-mm facial nerve defects.
Thirty-six Oryctolagus cuniculi were randomly divided into three groups (each group with 12 Oryctolagus cuniculi). In group A, chitosan conduit, collagen protein sponge, nerve growth factor (NGF), and NSCs were used. In group B, chitosan conduit, collagen sponge, and NGF were used. In group C, nerve autograft was performed. Electrophysiologic detection, histologic observation, and BrdU and S100 immunohistochemical examination were performed 12 weeks after operation.
All observation items in group A were better than those in group B (P < 0.01), and there were no significant differences between group A and group C (P > 0.05).
NSCs may be served as seed cells of peripheral nerve tissue engineering and be used in artificial nerve to repair facial nerve defects.
观察用于组织工程人工神经的神经干细胞（NSCs）修复兔10毫米面神经缺损的疗效。
36只穴兔随机分为三组（每组12只穴兔）。A组使用壳聚糖导管、胶原蛋白海绵、神经生长因子（NGF）和NSCs。B组使用壳聚糖导管、胶原海绵和NGF。C组进行自体神经移植。术后12周进行电生理检测、组织学观察以及BrdU和S100免疫组化检查。
A组所有观察指标均优于B组（P < 0.01），A组与C组之间无显著差异（P > 0.05）。
NSCs可作为周围神经组织工程的种子细胞，用于人工神经修复面神经缺损。
