建立一种灵敏且具选择性的液相色谱/串联质谱法，用于同时测定人滤泡性淋巴瘤细胞系中的细胞内1-β-D-阿拉伯呋喃糖基胞嘧啶三磷酸（araCTP）、胞苷三磷酸（CTP）和脱氧胞苷三磷酸（dCTP）。
Development of a sensitive and selective LC/MS/MS method for the simultaneous determination of intracellular 1-beta-D-arabinofuranosylcytosine triphosphate (araCTP), cytidine triphosphate (CTP) and deoxycytidine triphosphate (dCTP) in a human follicular lymphoma cell line.
作者信息
Crauste Céline, Lefebvre Isabelle, Hovaneissian Michael, Puy Jean Yves, Roy Béatrice, Peyrottes Suzanne, Cohen Sabine, Guitton Jérôme, Dumontet Charles, Perigaud Christian
机构信息
Institut des Biomolécules Max Mousseron, UMR 5247 CNRS-UM1-UM2, Université Montpellier 2, Montpellier Cedex 05, France.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 May 15;877(14-15):1417-25. doi: 10.1016/j.jchromb.2009.02.071. Epub 2009 Mar 17.
A method was developed for the quantification of araCTP, CTP and dCTP in a human follicular lymphoma cell line. This method involves solid phase extraction (SPE) using a weak anion-exchanger (WAX) cartridge, a porous graphitic carbon high-performance liquid chromatography (HPLC) column separation, and tandem mass spectrometry (MS/MS) detection. By using a triple quadrupole mass spectrometer operating in negative ion multiple reaction monitoring (MRM) mode, the method was able to achieve a lower limit of quantification (LLOQ) of 0.1 microg mL(-1) for araCTP and of 0.01 microg mL(-1) for both CTP and dCTP. The method was validated and used to determine the amount of araCTP, CTP and dCTP formed after incubation of araC and an araCMP prodrug in the human follicular lymphoma cell line RL.
开发了一种用于定量人滤泡性淋巴瘤细胞系中araCTP、CTP和dCTP的方法。该方法包括使用弱阴离子交换剂（WAX）柱进行固相萃取（SPE）、多孔石墨化碳高效液相色谱（HPLC）柱分离以及串联质谱（MS/MS）检测。通过使用在负离子多反应监测（MRM）模式下运行的三重四极杆质谱仪，该方法能够实现对araCTP的定量下限（LLOQ）为0.1 μg mL⁻¹，对CTP和dCTP均为0.01 μg mL⁻¹。该方法经过验证，并用于测定araC和一种araCMP前药在人滤泡性淋巴瘤细胞系RL中孵育后形成的araCTP、CTP和dCTP的量。
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