Klonisch Thomas, Glogowska Aleksandra, Gratao Ana A, Grzech Marjeta, Nistor Andreea, Torchia Mark, Weber Ekkehard, de Angelis Martin Hrabé, Rathkolb Birgit, Cuong Hoang-Vu, Wolf Eckhard, Schneider Marlon R
Department of Human Anatomy and Cell Science, University of Manitoba, 130-745 Bannatyne Avenue, Winnipeg, Canada.
FEBS Lett. 2009 Apr 17;583(8):1349-57. doi: 10.1016/j.febslet.2009.03.051. Epub 2009 Mar 27.
We generated transgenic mice to study the in vivo role of the cytoplasmic domain of human proEGF (proEGFcyt). Post-pubertal proEGFcyt transgenic (tg) mice displayed an up to 15% reduction in body weight, including smaller kidney and brain weights as compared to control littermates. Renal histology, gene expression profiles, and functional parameters were normal. In both sexes, serum levels of IGFBP-3 were reduced. Circulating IGF-I/IGF-II levels were unchanged. Histomorphological analysis revealed isolated foci of liver necrosis specific to proEGFcyt tg mice. In conclusion, we identified proEGF cytoplasmic domain as a novel modulator of whole body and organ-specific growth in mice.
我们构建了转基因小鼠以研究人proEGF胞质结构域（proEGFcyt）在体内的作用。青春期后的proEGFcyt转基因（tg）小鼠体重降低了15%，与同窝对照小鼠相比，肾脏和脑重量也较小。肾脏组织学、基因表达谱和功能参数均正常。两性的血清IGFBP - 3水平均降低。循环中的IGF - I/IGF - II水平未改变。组织形态学分析显示proEGFcyt tg小鼠肝脏出现特异性坏死灶。总之，我们确定proEGF胞质结构域是小鼠全身和器官特异性生长的新型调节因子。
