运用正相高效液相色谱法和液相色谱/质谱联用技术，从卡介苗东京172株（SMP - 105）及卡介苗亚菌株的细胞壁骨架中分离并进行分枝菌酸的分子表征分析。
Separation and molecular characterization of mycolic acid from the cell wall skeleton of Mycobacterium bovis BCG Tokyo 172 (SMP-105) and BCG substrains by normal-phase high performance liquid chromatography and liquid chromatography/mass spectrometry.
作者信息
Uenishi Yuko, Takii Takemasa, Yano Ikuya, Sunagawa Makoto
机构信息
Technology Research & Development Division, Dainippon Sumitomo Pharma Co., Ltd., 3-1-98, Kasugade-naka, Konohana-ku, Osaka 554-0022, Japan.
出版信息
J Microbiol Methods. 2009 Jun;77(3):320-2. doi: 10.1016/j.mimet.2009.03.006. Epub 2009 Mar 27.
PMID:19328829
Abstract
Since mycolic acids, the most characteristic major lipid component in mycobacterial cell envelopes, play pivotal roles in the cell surface-based host immune responses, normal-phase HPLC has been developed to quantify and identify mycolic acids of the cell wall skeleton from Mycobacterium bovis BCG Tokyo 172 (SMP-105).
摘要
由于分枝菌酸是分枝杆菌细胞壁包膜中最具特征的主要脂质成分，在基于细胞表面的宿主免疫反应中起关键作用，因此已开发出正相高效液相色谱法来定量和鉴定卡介苗东京172株（SMP-105）细胞壁骨架中的分枝菌酸。
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