通过分枝菌酸的高效液相色谱法从结核分枝杆菌和牛分枝杆菌中分离卡介苗（牛分枝杆菌卡介苗）
Separation of Mycobacterium bovis BCG from Mycobacterium tuberculosis and Mycobacterium bovis by using high-performance liquid chromatography of mycolic acids.
作者信息
Floyd M M, Silcox V A, Jones W D, Butler W R, Kilburn J O
机构信息
Division of Bacterial and Mycotic Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
出版信息
J Clin Microbiol. 1992 May;30(5):1327-30. doi: 10.1128/jcm.30.5.1327-1330.1992.
Abstract
Profile analysis of mycolic acid ester patterns of Mycobacterium tuberculosis, Mycobacterium bovis, and Mycobacterium bovis bacillus Calmette-Gúerin (BCG) using high-performance liquid chromatography indicated that separation of BCG from M. tuberculosis and M. bovis by elution and relative retention times is possible. Mycolic acid patterns of BCG eluted from the column 0.5 min before M. tuberculosis or M. bovis, resulting in relative retention times for two peaks not seen in the pattern of M. tuberculosis or M. bovis. Identification was confirmed by phage typing, which has been the standard procedure for confirmation of BCG strains. These results showed that high-performance liquid chromatographic analysis of mycolic acid esters can be used in the mycobacterial reference laboratory for separation of BCG from M. tuberculosis and M. bovis.
摘要
使用高效液相色谱法对结核分枝杆菌、牛分枝杆菌和卡介苗（BCG）的分枝菌酸酯模式进行的剖析分析表明，通过洗脱和相对保留时间可以将卡介苗与结核分枝杆菌和牛分枝杆菌分离。卡介苗的分枝菌酸模式在结核分枝杆菌或牛分枝杆菌之前0.5分钟从柱中洗脱，导致在结核分枝杆菌或牛分枝杆菌模式中未出现的两个峰的相对保留时间。通过噬菌体分型确认了鉴定结果，噬菌体分型一直是确认卡介苗菌株的标准程序。这些结果表明，分枝菌酸酯的高效液相色谱分析可用于分枝杆菌参考实验室，以将卡介苗与结核分枝杆菌和牛分枝杆菌分离。
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本文引用的文献
1
Identification of an effective vaccine against tuberculosis.
Am Rev Respir Dis. 1988 Aug;138(2):479-80. doi: 10.1164/ajrccm/138.2.479.
2
Identification of major slowly growing pathogenic mycobacteria and Mycobacterium gordonae by high-performance liquid chromatography of their mycolic acids.
J Clin Microbiol. 1988 Jan;26(1):50-3. doi: 10.1128/jcm.26.1.50-53.1988.
3
BCG identification by DNA restriction fragment patterns.
J Gen Microbiol. 1987 Jun;133(6):1431-4. doi: 10.1099/00221287-133-6-1431.
4
History of BCG and its substrains.
Prog Clin Biol Res. 1989;310:35-50.
5
High-performance liquid chromatography patterns of mycolic acids as criteria for identification of Mycobacterium chelonae, Mycobacterium fortuitum, and Mycobacterium smegmatis.
J Clin Microbiol. 1990 Sep;28(9):2094-8. doi: 10.1128/jcm.28.9.2094-2098.1990.
6
Identification of mycobacteria by high-performance liquid chromatography.
J Clin Microbiol. 1991 Nov;29(11):2468-72. doi: 10.1128/jcm.29.11.2468-2472.1991.
7
Differentiation of known strains of BCG from isolates of mycobacterium bovis and Mycobacterium tuberculosis by using mycobacteriophage 33D.
J Clin Microbiol. 1975 Apr;1(4):391-2. doi: 10.1128/jcm.1.4.391-392.1975.
8
Further studies of mycobacteriophage 33D (Warsaw) for differentiation of BCG from M. bovis and M. tubeculosis.
Tubercle. 1979 Mar;60(1):55-8. doi: 10.1016/0041-3879(79)90057-6.
9
Differentiation of BCG from other variants of Mycobacterium tuberculosis isolated from clinical material.
Tubercle. 1978 Jun;59(2):143-6. doi: 10.1016/0041-3879(78)90018-1.
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