皮栖固氮螺菌新种，从废弃焦油中分离得到。
Azospirillum picis sp. nov., isolated from discarded tar.
作者信息
Lin S-Y, Young C C, Hupfer H, Siering C, Arun A B, Chen W-M, Lai W-A, Shen F-T, Rekha P D, Yassin A F
机构信息
College of Agriculture and Natural Resources, Department of Soil & Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, ROC.
出版信息
Int J Syst Evol Microbiol. 2009 Apr;59(Pt 4):761-5. doi: 10.1099/ijs.0.65837-0.
A polyphasic taxonomic study was performed on a pink-coloured unknown bacterium isolated from discarded road tar. Comparative analysis of the 16S rRNA gene sequence demonstrated that the isolate belongs phylogenetically to the genus Azospirillum with Azospirillum lipoferum, A. melinis and A. rugosum as its closest phylogenetic relatives (96.7, 96.6 and 96.6 % similarity to the respective type strains). The generic assignment was confirmed on the basis of chemotaxonomic data, which revealed a fatty acid profile characteristic for the genus Azospirillum, consisting of straight-chain saturated and unsaturated fatty acids, with C(18 : 1)omega7c as the major unsaturated non-hydroxylated fatty acid, and C(16 : 0) 3-OH as the major hydroxylated fatty acid, and a ubiquinone with ten isoprene units (Q-10) as the predominant respiratory quinone. On the basis of both the phenotypic and molecular genetic evidence, it is proposed that the unknown isolate should be classified within a novel species of the genus Azospirillum, for which the name Azospirillum picis sp. nov. is proposed. The type strain is IMMIB TAR-3(T) (=CCUG 55431(T) =DSM 19922(T)).
对从废弃道路焦油中分离出的一种粉红色未知细菌进行了多相分类学研究。16S rRNA基因序列的比较分析表明，该分离株在系统发育上属于固氮螺菌属，与产脂固氮螺菌、黑麦草固氮螺菌和粗糙固氮螺菌是最接近的系统发育亲缘关系（与相应模式菌株的相似性分别为96.7%、96.6%和96.6%）。基于化学分类学数据证实了该属的归属，该数据揭示了固氮螺菌属特有的脂肪酸谱，由直链饱和脂肪酸和不饱和脂肪酸组成，以C(18 : 1)ω7c作为主要的不饱和非羟基脂肪酸，C(16 : 0) 3-OH作为主要的羟基脂肪酸，以及以含有十个异戊二烯单元的泛醌（Q-10）作为主要呼吸醌。基于表型和分子遗传学证据，建议将该未知分离株归类为固氮螺菌属的一个新物种，为此提出名为皮氏固氮螺菌（Azospirillum picis sp. nov.）。模式菌株为IMMIB TAR-3(T)（=CCUG 55431(T) =DSM 19922(T)）。
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