Zhang Liefeng, Jiang Hui, Zhu Wenjie, Wu Lin, Song Lingling, Wu Qiuyan, Ren Yong
Jiangsu Key Laboratory for Supramolecular Medicinal and Applications, College of Life Science, Nanjing Normal University, Nanjing 210097, P.R. China.
Int J Mol Sci. 2008 Dec;9(12):2376-2387. doi: 10.3390/ijms9122376. Epub 2008 Dec 1.
Degradation of insulin was studied in this work. Casein and protamine could obviously suppress degradation of insulin by intestinal enzymes, and could protect insulin from degradation by the mechanism of competition and combination with proteolysis enzyme. What is more, co-incubated with HP-beta-CD-casein or HP-beta-CD-protamine, most insulin was protected from degradation by intestinal enzymes. In addition, it was found that the complexation of insulin with HP-beta-CD was characterized by UV absorption spectra. These results indicated that HP-beta-CD, casein and protamine could offer some positive and useful results, and could protect insulin from degradation during their transit through the intestinal tract.
本研究对胰岛素的降解情况进行了探讨。酪蛋白和鱼精蛋白能够显著抑制肠道酶对胰岛素的降解作用，并且可通过与蛋白水解酶竞争结合的机制来保护胰岛素不被降解。此外，与HP-β-环糊精-酪蛋白或HP-β-环糊精-鱼精蛋白共同孵育时，大多数胰岛素可免受肠道酶的降解。另外，研究发现胰岛素与HP-β-环糊精的络合作用可通过紫外吸收光谱进行表征。这些结果表明，HP-β-环糊精、酪蛋白和鱼精蛋白能够提供一些积极且有益的结果，并且在胰岛素通过肠道的过程中能够保护其不被降解。
