The Conductive and Electroactive Polymers Research Unit, The Petroleum and Petrochemical College, Chulalongkorn University, Bangkok, Thailand.
Division of Perioperative and Ambulatory Medicine, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Drug Deliv. 2022 Dec;29(1):2234-2244. doi: 10.1080/10717544.2022.2096717.
Insulin is the peptide hormone used to treat the diabetes patient. The hormone is normally taken by injection. The transdermal drug delivery system (TDDS) is an alternative route. The silk fibroin (SF) hydrogels were fabricated via solution casting as the insulin matrix. The release and release-permeation experiments of the insulin loaded SF hydrogels were carried out using a modified Franz-diffusion cell at 37 °C for 36 h, under the effects of SF concentrations, pH, and electric field. The release-permeation mechanism through the pig skin was from the Case-II transport with the constant release rate. The diffusion coefficient (D) increased with decreasing SF concentration due to a larger mesh size, and with increasing electric field due to the electroreplusive forces between the insulin and the SF hydrogels against the negatively-charged electrode, and the induced SF hydrogel expansion. The rate and amount of insulin release-permeation became relatively lower as it required a longer time to generate aqueous pathways through the pig skin. The present SF hydrogels are demonstrated here deliver insulin with the required constant release rate, and the suitable amount within a prescribed duration.
胰岛素是用于治疗糖尿病患者的肽类激素。该激素通常通过注射给药。透皮给药系统（TDDS）是一种替代途径。丝素（SF）水凝胶是通过溶液浇铸作为胰岛素基质来制备的。在 37°C 下，使用改良的 Franz 扩散池，在 SF 浓度、pH 值和电场的影响下，进行了负载胰岛素的 SF 水凝胶的释放和渗透实验，持续 36 小时。通过猪皮的释放渗透机制是具有恒速释放的 Case-II 传输。扩散系数（D）随着 SF 浓度的降低而增加，这是由于较大的网孔尺寸，随着电场的增加，这是由于胰岛素和 SF 水凝胶之间的电排斥力以及对带负电荷的电极的 SF 水凝胶的诱导膨胀。胰岛素释放渗透的速率和量变得相对较低，因为需要更长的时间才能在猪皮中产生水通路。这里展示的 SF 水凝胶被证明可以以所需的恒速释放胰岛素，并在规定的时间内释放适量的胰岛素。
