Belz Regina G, Hurle Karl, Duke Stephen O
University of Hohenheim, Institute of Phytomedicine 360, Department of Weed Science, Stuttgart, Germany.
Nonlinearity Biol Toxicol Med. 2005 Apr;3(2):173-211. doi: 10.2201/nonlin.003.02.002.
The response of an organism to a chemical depends, among other things, on the dose. Nonlinear dose-response relationships occur across a broad range of research fields, and are a well established tool to describe the basic mechanisms of phytotoxicity. The responses of plants to allelochemicals as biosynthesized phytotoxins, relate as well to nonlinearity and, thus, allelopathic effects can be adequately quantified by nonlinear mathematical modeling. The current paper applies the concept of nonlinearity to assorted aspects of allelopathy within several bioassays and reveals their analysis by nonlinear regression models. Procedures for a valid comparison of effective doses between different allelopathic interactions are presented for both, inhibitory and stimulatory effects. The dose-response applications measure and compare the responses produced by pure allelochemicals [scopoletin (7-hydroxy-6-methoxy-2H-1-benzopyran-2-one); DIBOA (2,4-dihydroxy-2H-1,4-benzoxaxin-3(4H)-one); BOA (benzoxazolin-2(3H)-one); MBOA (6-methoxy-benzoxazolin-2(3H)-one)], involved in allelopathy of grain crops, to demonstrate how some general principles of dose responses also relate to allelopathy. Hereupon, dose-response applications with living donor plants demonstrate the validity of these principles for density-dependent phytotoxicity of allelochemicals produced and released by living plants (Avena sativa L., Secale cereale L., Triticum L. spp.), and reveal the use of such experiments for initial considerations about basic principles of allelopathy. Results confirm that nonlinearity applies to allelopathy, and the study of allelopathic effects in dose-response experiments allows for new and challenging insights into allelopathic interactions.
生物体对化学物质的反应，在其他因素中，取决于剂量。非线性剂量反应关系出现在广泛的研究领域，是描述植物毒性基本机制的一种成熟工具。植物对作为生物合成植物毒素的化感物质的反应，也与非线性相关，因此，化感效应可以通过非线性数学建模进行充分量化。本文将非线性概念应用于多种生物测定中的化感作用的各个方面，并通过非线性回归模型揭示其分析方法。针对抑制和刺激作用，都提出了有效比较不同化感相互作用之间有效剂量的程序。剂量反应应用测量并比较了参与谷类作物化感作用的纯化感物质[东莨菪素（7-羟基-6-甲氧基-2H-1-苯并吡喃-2-酮）；DIBOA（2,4-二羟基-2H-1,4-苯并恶唑-3(4H)-酮）；BOA（苯并恶唑啉-2(3H)-酮）；MBOA（6-甲氧基-苯并恶唑啉-2(3H)-酮）]产生的反应，以证明剂量反应的一些一般原则也与化感作用相关。据此，使用活体供体植物的剂量反应应用证明了这些原则对于活体植物（燕麦、黑麦、小麦属）产生和释放的化感物质的密度依赖性植物毒性的有效性，并揭示了此类实验在化感作用基本原理初步思考中的应用。结果证实非线性适用于化感作用，并且在剂量反应实验中研究化感效应能够为化感相互作用带来新的、具有挑战性的见解。
