Zhou Weibin, Hildebrandt Friedhelm
Departments of Pediatrics and Human Genetics, University of Michigan, 8220 MSRB3, 1150 W Medical Center Drive, Ann Arbor, MI 48109-5646, USA.
Gene Expr Patterns. 2009 Jun;9(5):282-8. doi: 10.1016/j.gep.2009.03.003. Epub 2009 Mar 28.
Phospholipase C (PLC) is a key enzyme that generates inositol 1,4,5-triphosphate (IP(3)) and diacylglycerol (DAG), two second messengers in signal transduction. Phospholipase Cepsilon (PLCE1) is a unique member of the PLC family in that it carries both RasGEF and Ras-associating (RA) domains and may serve as an activator and an effector of small GTPases. Recently, mutations in PLCE1 have been associated with early-onset nephrotic syndrome. We have identified the zebrafish ortholog of PLCE1 and characterized its expression pattern in zebrafish embryos by in situ hybridization. Zebrafish plce1 gene encodes a protein of 2248 amino acids that shares 56% identity with mammalian PLCepsilon. During zebrafish embryonic development, plce1 is expressed in a dynamic pattern in a variety of organs, such as brain, muscle, liver, and pronephric glomeruli. Our results suggest that zebrafish may be used as a model organism to address the function of PLCepsilon during the development of these organs.
磷脂酶C（PLC）是一种关键酶，可生成肌醇1,4,5-三磷酸（IP(3)）和二酰基甘油（DAG），这两种是信号转导中的第二信使。磷脂酶Cε（PLCE1）是PLC家族中的独特成员，因为它同时具有RasGEF和Ras结合（RA）结构域，并且可能作为小GTP酶的激活剂和效应器。最近，PLCE1中的突变与早发性肾病综合征有关。我们已经鉴定出斑马鱼中PLCE1的直系同源物，并通过原位杂交表征了其在斑马鱼胚胎中的表达模式。斑马鱼plce1基因编码一个2248个氨基酸的蛋白质，与哺乳动物的PLCε有56%的同源性。在斑马鱼胚胎发育过程中，plce1在多种器官中以动态模式表达，如脑、肌肉、肝脏和前肾肾小球。我们的结果表明，斑马鱼可作为一种模式生物来研究PLCε在这些器官发育过程中的功能。
