Moro Enrico, Maran Claudio, Slongo M Liliana, Argenton Francesco, Toppo Stefano, Onisto Maurizio
Dept. of Biology, University of Padua, Padova, Italy.
Int J Dev Biol. 2007;51(3):241-6. doi: 10.1387/ijdb.062220em.
Spata2 (spermatogenesis-associated protein 2) was originally described as a novel gene involved in the spermatogenic process. In this study, we cloned a potential zebrafish spata2 orthologue. The consensus open reading frame (1650 bp) encodes a polypeptide of 550 amino acids which shares 37% identity with the human SPATA2. Bioinformatic analysis reveals a small pattern PW [KR] KE [YF][RK] which seems to be of particular interest in the light of its strong conservation between SPATA2 and the recently discovered TAMO protein of D. melanogaster. RT-PCR analysis in adult zebrafish tissues revealed that spata2 mRNA has a broad distribution. Whole-mount in situ hybridization demonstrated that spata2 transcripts are maternally derived and becomes strongly localized in the central nervous system at early developmental stages. From 5 dpf, spata2 expression becomes detectable in the gut and pronephric duct epithelium, suggesting a wide tissue function during vertebrate development.
Spata2（精子发生相关蛋白2）最初被描述为一个参与精子发生过程的新基因。在本研究中，我们克隆了一个潜在的斑马鱼spata2直系同源基因。共有开放阅读框（1650 bp）编码一个由550个氨基酸组成的多肽，该多肽与人类SPATA2具有37%的同一性。生物信息学分析揭示了一个小模式PW [KR] KE [YF][RK]，鉴于其在SPATA2和最近发现的黑腹果蝇TAMO蛋白之间的高度保守性，该模式似乎特别值得关注。对成年斑马鱼组织进行的RT-PCR分析表明，spata2 mRNA分布广泛。整体原位杂交显示，spata2转录本是母源的，并在发育早期强烈定位于中枢神经系统。从5日龄仔鱼开始，在肠道和前肾管上皮中可检测到spata2的表达，这表明其在脊椎动物发育过程中具有广泛的组织功能。
